Depends a bit on what the "streaks" look like. If they are curved, and
your "streaks" are connecting partial rings at different resolutions,
then what you may be seeing is simply diffuse scatter from a "small
molecule", such as ice. The only way to be sure you are looking at
protein is if you
- improve the
crystal or improve the freezing conditions.
Mark
-Original Message-
From: Demetres D. Leonidas
To: CCP4BB
Sent: Thu, Oct 3, 2013 7:05 am
Subject: Re: [ccp4bb] X-ray pattern has streaks
Dear Krithika,
what you probably see is the diffraction pattern of a highly
One standard thing to do would be to set up a grid around the condition and
then try picking crystals from different well to see if any of them produce
better looking diffraction patterns. If that does not work one might give
any of the commercially available additive screen a try to see if
diffrac
Dear Krithika,
what you probably see is the diffraction pattern of a highly mosaic
crystal due to probably internal disordering.
cheers
Demetres
On 3/10/2013 3:40 μμ, Krithika GOkulnath wrote:
I introduce myself as Dr.Krithika Gokulnath, CAS in crystallography and
Biophysics, UNiversity o
I introduce myself as Dr.Krithika Gokulnath, CAS in crystallography and
Biophysics, UNiversity of Madras. I have been working with a cloned protein and
have been successfully able to crystallise it. However data collection done at
home source shows a pattern of streaks instead of spots. The crys
