A
Ronau
Sent: 15 January 2013 14:47
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] crystal dehydration
Greetings!
I have recently been attempting crystal dehydration experiments to improve
diffraction following the procedures from the ERSF in which crystals are
exposed to increased concentrations
-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Hargreaves, David
Sent: 16 January 2013 12:37
To: ccp4bb
Subject: Re: [ccp4bb] crystal dehydration
Judith,
If you can, try monitoring the diffraction during the dehydration experiment.
There are plenty of ways of doing
Greetings!
I have recently been attempting crystal dehydration experiments to improve
diffraction following the procedures from the ERSF in which crystals are
exposed to increased concentrations of precipitant. I would like to know if
anyone knew of any alternative methods for dehydration of
Dear Judith,
in Acta Cryst D61 (2005), 1173-1180 different handling methods for dehydration
are described besides other post-crystallization treatments to improve
diffraction quality.
You can also try Dehydration Salts. A screen with ready made salt solutions is
available from Jena Bioscience.
Beamline Scientist - I02
Tel: +44 (0)1235 778661
Mob:+44 (0)7795 641259
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Judith A
Ronau
Sent: 15 January 2013 14:47
To: ccp4bb
Subject: [ccp4bb] crystal dehydration
Hi Judith,
a very effective method is the use of a humidity control device - this
was actually the reason we developed the equations that predict the RH
in equilibrium with precipitant solutions. It has the great advantage
that you can characterize changes that occur and also move straight to
In case if dehydration needs to be done slowly and under tight control of all
parameters, one possibility is to use micro-dialysis buttons.
We used it for a large membrane protein complex and diffraction improved from
~7 to 2.7 A. The crystal is fished out and put into mother liquor solution
Hi Partha,
as far as I know the HC1 is available at the CLS and soon at the APS
and ALS, best wishes, Matthew.
On 2013-01-15 22:27, Parthasarathy Sampathkumar wrote:
Dear Dr. Bowler,
Which beamline in USA has this Humidity Control Device?
Best Wishes,
Partha
On Tue, Jan 15, 2013 at 11:02
