Hi Judith,
a very effective method is the use of a humidity control device - this
was actually the reason we developed the equations that predict the RH
in equilibrium with precipitant solutions. It has the great advantage
that you can characterize changes that occur and also move straight to
data collection. There are several HC1 devices (developed here at the
EMBL) in Europe and at least 1 in the USA - there is also the FMS. Below
are sole links that might help, best wishes, Matt.
Website for experiments:
http://www.esrf.eu/UsersAndScience/Experiments/MX/About_our_beamlines/ID14-2/HC1b
Calculation server for mother liquor RH equilibria:
http://go.esrf.eu/RH
Paper describing above:
http://scripts.iucr.org/cgi-bin/paper?S1744309111054029
Papers describing device and methods:
http://journals.iucr.org/d/issues/2009/12/00/gm5010/index.html and
http://www.sciencedirect.com/science/article/pii/S1047847711000499
Interface in MXCuBE that allows the design of dehydration gradients,
data collection and analysis of the images
http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/workflows/dehydration-workflow.
On 2013-01-15 15:46, Judith A Ronau wrote:
Greetings!
I have recently been attempting crystal dehydration experiments to
improve diffraction following the procedures from the ERSF in which
crystals are exposed to increased concentrations of precipitant. I
would like to know if anyone knew of any alternative methods for
dehydration of protein crystals. Thanks!
Best,
Judith
--
Matthew Bowler
Synchrotron Science Group
European Molecular Biology Laboratory
BP 181, 6 rue Jules Horowitz
38042 Grenoble Cedex 9
France
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Tel: +33 (0) 4.76.20.76.37
Fax: +33 (0) 4.76.88.29.04
http://www.embl.fr/
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