Hi Partha,
as far as I know the HC1 is available at the CLS and soon at the APS
and ALS, best wishes, Matthew.
On 2013-01-15 22:27, Parthasarathy Sampathkumar wrote:
Dear Dr. Bowler,
Which beamline in USA has this Humidity Control Device?
Best Wishes,
Partha
On Tue, Jan 15, 2013 at 11:02 AM, Matthew BOWLER <[email protected]
[10]> wrote:
Hi Judith,
a very effective method is the use of a humidity control device -
this was actually the reason we developed the equations that predict
the RH in equilibrium with precipitant solutions. It has the great
advantage that you can characterize changes that occur and also move
straight to data collection. There are several HC1 devices
(developed here at the EMBL) in Europe and at least 1 in the USA -
there is also the FMS. Below are sole links that might help, best
wishes, Matt.
Website for experiments:
http://www.esrf.eu/UsersAndScience/Experiments/MX/About_our_beamlines/ID14-2/HC1b
[1]
Calculation server for mother liquor RH equilibria:
http://go.esrf.eu/RH [2]
Paper describing above:
http://scripts.iucr.org/cgi-bin/paper?S1744309111054029 [3]
Papers describing device and methods:
http://journals.iucr.org/d/issues/2009/12/00/gm5010/index.html [4]
and
http://www.sciencedirect.com/science/article/pii/S1047847711000499
[5]
Interface in MXCuBE that allows the design of dehydration
gradients, data collection and analysis of the images
http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/workflows/dehydration-workflow
[6].
On 2013-01-15 15:46, Judith A Ronau wrote:
Greetings!
I have recently been attempting crystal dehydration experiments
to
improve diffraction following the procedures from the ERSF in
which
crystals are exposed to increased concentrations of precipitant.
I
would like to know if anyone knew of any alternative methods for
dehydration of protein crystals. Thanks!
Best,
Judith
--
Matthew Bowler
Synchrotron Science Group
European Molecular Biology Laboratory
BP 181, 6 rue Jules Horowitz
38042 Grenoble Cedex 9
France
===================================================
Tel: +33 (0) 4.76.20.76.37 [7]
Fax: +33 (0) 4.76.88.29.04 [8]
http://www.embl.fr/ [9]
===================================================
Links:
------
[1]
http://www.esrf.eu/UsersAndScience/Experiments/MX/About_our_beamlines/ID14-2/HC1b
[2] http://go.esrf.eu/RH
[3] http://scripts.iucr.org/cgi-bin/paper?S1744309111054029
[4] http://journals.iucr.org/d/issues/2009/12/00/gm5010/index.html
[5]
http://www.sciencedirect.com/science/article/pii/S1047847711000499
[6]
http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/workflows/dehydration-workflow
[7] http://embl.fr/tel:%2B33%20%280%29%204.76.20.76.37
[8] http://embl.fr/tel:%2B33%20%280%29%204.76.88.29.04
[9] http://www.embl.fr/
[10] mailto:[email protected]
--
Matthew Bowler
Synchrotron Science Group
European Molecular Biology Laboratory
BP 181, 6 rue Jules Horowitz
38042 Grenoble Cedex 9
France
===================================================
Tel: +33 (0) 4.76.20.76.37
Fax: +33 (0) 4.76.88.29.04
http://www.embl.fr/
===================================================
