Ursula,
Most compounds used for cryosolutions glycerol, ethylene glycol, propane
diol increase protein solubility.
A warning, these compounds are also hygroscopic, you need to change your
vapour diffusion methodology.
Vera L., Czarny B., Georgiadis D., Dive V., Stura E.A. (2011) Practical
of Enrico
Stura [est...@cea.fr]
Sent: 20 February 2015 14:36
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] how to reduce protein solubility
Ursula,
Most compounds used for cryosolutions glycerol, ethylene glycol, propane
diol increase protein solubility.
A warning, these compounds are also
] *On Behalf Of *
Mattiroli,Francesca
*Sent:* Tuesday, February 17, 2015 5:23 AM
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] how to reduce protein solubility
Hi all,
I am struggling with a protein complex that is too soluble. I have reached
about 20 mg/ml but I still observe very little
.
Alun
Sent from my Samsung Galaxy smartphone.
Original message
From: Mattiroli,Francesca francesca.mattir...@colostate.edu
Date:17/02/2015 04:34 (GMT+00:00)
To: CCP4BB@JISCMAIL.AC.UK
Cc:
Subject: [ccp4bb] how to reduce protein solubility
Hi all,
I am struggling with a protein
+00:00)
To: CCP4BB@JISCMAIL.AC.UK
Cc:
Subject: [ccp4bb] how to reduce protein solubility
Hi all,
I am struggling with a protein complex that is too soluble. I have reached
about 20 mg/ml but I still observe very little precipitation (clear drops in
90-95% of the tested conditions). The proteins
Thank you, everyone!
Francesca
From: Enrico Stura [est...@cea.fr]
Sent: Tuesday, February 17, 2015 3:00 AM
To: CCP4BB@jiscmail.ac.uk; Mattiroli,Francesca
Subject: Re: [ccp4bb] how to reduce protein solubility
Francesca,
The most common failure is to have
Hi Francesca,
Try some zinc (1mM + ) in your protein buffer? Zinc tends to make a lot of
things less soluble in my hands.
Dave
On Tue, 17 Feb 2015 04:34 Mattiroli,Francesca
francesca.mattir...@colostate.edu wrote:
Hi all,
I am struggling with a protein complex that is too soluble. I have
Briggs
Sent: 17 February 2015 08:12
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] how to reduce protein solubility
Hi Francesca,
Try some zinc (1mM + ) in your protein buffer? Zinc tends to make a lot of
things less soluble in my hands.
Dave
On Tue, 17 Feb 2015 04:34 Mattiroli,Francesca
Francesca,
The most common failure is to have an excessive amount of salt (salting
in/ salting out), glycerol or other solubilizing
ingredient in your protein solution. I would suggest that you change the
pH and reduce the salt in your protein solution,
by microdialysis if you do not have
Of
Mattiroli,Francesca
Sent: Tuesday, February 17, 2015 5:23 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] how to reduce protein solubility
Hi all,
I am struggling with a protein complex that is too soluble. I have reached
about 20 mg/ml but I still observe very little precipitation (clear drops
Bringing the pH closer to the measured pI would definitely help. The pI can
be measured on an IEF gel. Glycosylation, if applicable, could dramatically
increase solubility.
Roger Rowlett
On Feb 16, 2015 11:33 PM, Mattiroli,Francesca
francesca.mattir...@colostate.edu wrote:
Hi all,
I am
Hi all,
I am struggling with a protein complex that is too soluble. I have reached
about 20 mg/ml but I still observe very little precipitation (clear drops in
90-95% of the tested conditions). The proteins are expressed in insect cells
and going to higher concentration is not easily
, 2015 11:23 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] how to reduce protein solubility
Hi all,
I am struggling with a protein complex that is too soluble. I have reached
about 20 mg/ml but I still observe very little precipitation (clear drops in
90-95% of the tested conditions
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