Re: [ccp4bb] temperature after 30 minutes using microscopes ?

2009-02-05 Thread Jürgen Bosch
Here's the summary and some own experimental data. First off all thank you all for your replies. trick 1: put a glass bottle filled with water between fiberoptics and the mirrors of your base (Isolde Le Trong) trick 2: suspend plates to allow air circulation, with bulb under plate scopes (Jo

Re: [ccp4bb] temperature after 30 minutes using microscopes ?

2009-01-30 Thread Jeffrey Wilson
On Jan 21, 2009, at 6:35 PM, matthew.frank...@imclone.com wrote: My light source is halogen, but it's coupled to the microscope through a fiber optic light pipe. I know from experience that the older model scopes with the bulb right in the base get quite warm after they've been on for a whi

Re: [ccp4bb] temperature after 30 minutes using microscopes ?

2009-01-22 Thread Sandy, DJ (James)
We have both LED and cold light sources attached to our Zeiss Stemi microscopes on the beamlines at Diamond. There was some argument about not using LED lights however the solution from Zeiss is very good allowing different sections of LEDs to be used which can be moved around. This allows crystal

Re: [ccp4bb] temperature after 30 minutes using microscopes ?

2009-01-21 Thread Clemens Grimm
We have tested the Zeiss LED plate with hanging and sitting drop trays and found it it unsuitable for looking at crystals. The reason is the really low contrast with this kind of diffuse illumination. Obviously, contrast is generated by refraction at crystal/mother liquor interfaces and thi

Re: [ccp4bb] temperature after 30 minutes using microscopes ?

2009-01-21 Thread Jürgen Bosch
Here are some numbers Matthew.Franklin: Okay. Start: 71.9 F Finish: 73.1 F Temperature measured with thermocouple Scotch-taped to the center of the microscope stage, underneath an empty 96-well plate. Room thermostat set to 70. I should have mentioned that I was thinking about fibero

Re: [ccp4bb] temperature after 30 minutes using microscopes ?

2009-01-21 Thread Petr Leiman
ot;Jürgen Bosch" To: Sent: Wednesday, January 21, 2009 11:50 PM Subject: [ccp4bb] temperature after 30 minutes using microscopes ? Hi there, *warning, reading beyond this line might expose you to non CCP4 related topics* anybody out there who could do the following experiment: Turn on your mi

Re: [ccp4bb] temperature after 30 minutes using microscopes ?

2009-01-21 Thread Joao Dias
Jurgen, From your question I assume that you are having problems with the warming up of the crystal tray due to the microscope light. Did you consider the use of an external cold light source like the KL1500 LCD? I do not feel anything warming up. Which microscope are you using? The LED sy

Re: [ccp4bb] temperature after 30 minutes using microscopes ?

2009-01-21 Thread Matthew . Franklin
CCP4 bulletin board wrote on 01/21/2009 05:50:13 PM: > Hi there, > *warning, reading beyond this line might expose you to non CCP4 > related topics* > > anybody out there who could do the following experiment: > Turn on your microscope and measure the temperature after 30 minutes > where you woul

[ccp4bb] temperature after 30 minutes using microscopes ?

2009-01-21 Thread Jürgen Bosch
Hi there, *warning, reading beyond this line might expose you to non CCP4 related topics* anybody out there who could do the following experiment: Turn on your microscope and measure the temperature after 30 minutes where you would place your precious crystal tray. In particular I'm intere