rsday, August 1, 2019 6:29 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Importance of temperature during initial crystallization
screening
Interesting topic,
Certainly the two papers suggested by Georg are relevant, and I fully agree
with the comments from Daniel that it is hard to predict
Dear Sergei,
There is also this high temperature crystallisation study of lysozyme, up to 55
degrees C:-
https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2872%2980273-4
Best wishes,
John
Emeritus Professor John R Helliwell DSc
> On 1 Aug 2019, at 10:23, Sergei Strelkov wrote:
>
Campus, Cardiff, CF14 4XN
>>
>> email: rizkall...@cardiff.ac.uk <mailto:rizkall...@cardiff.ac.uk>
>> phone: +44 29 2074 2248
>>
>> http://www.cardiff.ac.uk/people/view/126690-rizkallah-pierre
>> <http://www.cardiff.ac.uk/people/view/126
etin board On Behalf Of Georg Mlynek
Sent: Friday, 2 August 2019 5:09 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Importance of temperature during initial crystallization
screening
Hi Sergei, this publication should be useful for you.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC475
@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Importance of temperature during initial crystallization
screening
Hi Sergei, this publication should be useful for you.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756611/
Additionally it is proposed that when your protein is not so stable (lower Tm),
one should
Sergei,
That depends heavily on the protein, so I don't know if a statistical study
will accurately reflect the
importance of temperature. With some proteins, no matter how thoroughly
you sample chemical
space, temperature is critical, and a study would have to focus on such a
protein. The data
Hi Sergei
We did some data-mining on this way, way back, in 2004.
See the second section in this link
https://www.douglas.co.uk/PDB_data.htm
When you consider the *non-standard *temps - ie NOT 4C or 20C - it *looks*
as though the higher-end temps *may *work better. But of course it's hard
to
