Is the R factor for the best solution really > 40% - if so there must be
some major errors to correct.
Eleanor
On Fri, 31 Jul 2020 at 11:08, John R Helliwell
wrote:
> Dear Rafal,
> The difference map you show in your attachment has an echo of the main
> structure sort of look. So, I suggest you
Dear Rafal,
The difference map you show in your attachment has an echo of the main
structure sort of look. So, I suggest you examine your diffraction images
specifically looking out for a supercell of weak intensity spots, not picked up
by the autoindexing, between the main lattice of
Dear Rafal,
with such a small molecule, and if you really have 1.0A resolution, you
should be able to solve the structure by direct methods with shelxt. By
default, shelxt searches all space groups in the Laue group. The output
will not be very practical to assign residue, etc. but at least it
-Ursprüngliche Nachricht-
Von: CCP4 bulletin board Im Auftrag von Rafal Dolot
Gesendet: Donnerstag, 30. Juli 2020 14:06
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [EXTERNAL] Re: [ccp4bb] Problems with refinement of nucleic acid
structure
EXTERNAL : Real sender is owner-ccp...@jiscmail.ac.uk
Dear all,
Thank you for the response. I will try to explain it more precisely.
The molecule of interest is a duplex with 9 nt length, but is paired on
the length of eight bases, with overhangs at ends. Molecules form a
parallel strings across the crystal lattice, parallel to C-axis, because
