Vandu Murugan wrote:
Dear all,
I have collected a 2.7 angstrom home source data with Cu-Kalpha
source for a protein with 6 cysteines, with a multiplicity of around
23. I need to know, is there any significant anamolous signal present
in the data set, since there is no good model for my
Dear Murugan,
you can use the program hkl2map from Thomas Schneider, available at
http://webapps.embl-hamburg.de/hkl2map/
It's a graphical interface to the programs shelx c/d/e which are available from
http://shelx.uni-ac.gwdg.de/SHELX/index.html
With SAD data you want to look at the d/sigma
Hi Murugan,
One useful indicator of raw anomalous signal is the ANOMPLOT graph
from Scala - this shows the differences between reflections compared
with the expected differences. If the gradient of the plot is 1
there's no more differences that you would expect. If the gradient is
more than one
Dear Murugan,
Am 29.06.10 11:05, schrieb Vandu Murugan:
Dear all,
I have collected a 2.7 angstrom home source data with Cu-Kalpha
source for a protein with 6 cysteines, with a multiplicity of around
23. I need to know, is there any significant anamolous signal present
in the data set,
I've found the scala CC-anom significantly underestimates the anomalous
signal, relative to e.g. xprep. I don't know why that is, but the
latter seems to agree with what shelxd is happy with.
Cheers
phx
On 29/06/2010 10:35, Graeme Winter wrote:
Hi Murugan,
One useful indicator of raw
I would say CC-anom 0.3 or even 0.2 (note that the scala CC-anom is defined
on I not F)
Phil
On 29 Jun 2010, at 14:37, Frank von Delft wrote:
I've found the scala CC-anom significantly underestimates the anomalous
signal, relative to e.g. xprep. I don't know why that is, but the latter
Answering the question should I even bother trying? can be
complicated, but I get asked that a lot at the beamline! I recently
incorporated a number of data quality formulas into a little interactive
web page here:
http://bl831.als.lbl.gov/xtalsize.html
which focuses on calculating how many
On Tue, Jun 29, 2010 at 09:30:30AM -0700, James Holton wrote:
Answering the question should I even bother trying? can be
complicated, [...]
-James Holton
MAD Scientist
Since 'trying' may only take a semi-experienced user about 30min until they
could have a poly-Alanine trace of their
parameters.
The hkl2map graphs are enormously helpful for this purpose.
BR
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Tim
Gruene
Sent: Tuesday, June 29, 2010 2:29 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] measure of anamolous signal
Dear
