1, Check your directory permission. Make sure you have the read and execute
permission for that directory (sometimes like this* drwxr-xr-x*).
2, Try to install CCP4 package by *fink*. There's already a stable version
for Lion. (http://pdb.finkproject.org/pdb/package.php/ccp4)
On Tue, May 22,
lovely density - i would like to know what % of series have multiple
conformations - it would need a survey I guess of PDB depositions, not just
coordinates, but checking maps..
Eleanor
On 21 May 2012 22:21, Uma Ratu rosiso2...@gmail.com wrote:
Thank you All for you inputs.
Uma
On Mon,
Dear Uma
1. The protein sequence given in the databases, do from time to time
have errors, particularly if you are working with old proteins (when
the sequencing was done a long time ago). What you observe could also be
a threonine or even a valine. You should check whether homologous
3. Of course if you are working on recombinant protein, you should
double check the sequencing results from the cloning
Preben
On 5/21/12 11:21 PM, Uma Ratu wrote:
Thank you All for you inputs.
Uma
On Mon, May 21, 2012 at 5:06 PM, Van Den Berg, Bert
lambertus.vandenb...@umassmed.edu
Hi folks
The list of microsymposia at ECM27 is now available, but the list of speakers
has not yet been finalised. Bear in mind that earlybird registration closes
on 31st May, when the cost jumps by 750 NOK (~€100) for full participants,
550NOK (~€72) for students.
The meeting website is
thanks everybody.
On Mon, May 21, 2012 at 1:51 PM, mjvdwo...@netscape.net wrote:
There is a really nice web site that shows how colors are perceived by
various color-blind readers. One of the journals I recently published in
recommends it for consideration. If you are interested, have a look.
Dear CCP4ers,
I am working on purifying a protein-DNA complex for structural and
biochemical studies. So far, I can readily make protein 95% pure in high
salt buffer. However, I have some problems in assembling the protein-DNA
complex.
1) My protein precipitates at low salt buffer. I think this
Wei,
I am not sure if I can solve your problem, but I can try to give insight.
I am also working with a similar protein-DNA complex situation. I normally
purify the protein by itself, and maintain the salt at 250 mM or greater.
Addition of 1.5x DNA stabilizes this complex and I am able to
The group of Dr. Marcin Nowotny at the International Institute of Molecular and
Cell Biology (IIMCB) in Warsaw, Poland is seeking candidates for postdoctoral
fellows. The fellows will work on protein complexes involved in DNA and RNA
metabolism using protein crystallography and protein
Hello everyone,
My apologies if this comes as basic, but I wanted to get the expert's take on
whether or not the sigmaF values are required in the calculation of an electron
density map. If I look at the standard ED equation, sigma's don't appear to be
a requirement but all the scripts that
Your understanding is correct, sigmaF values aren't required for
calculating electron density.
Many programs that calculate maps have an option to use the F/sigmaF
ratio to threshold the amplitudes used in map calculation - which would
require sigmaF. This isn't something I've seen used
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