*** DEADLINE JUNE 27TH ***
I would like to draw your attention to a current opening in my laboratory, to
work on the electron microscopy of CRISPR complexes with a single particle
approach (see Zhang et al., Mol. Cell 2012).
The project is really quite exciting. We have plenty of great
Dear colleagues,
is there any program enclosed in CCP4 to convert a *.brix file or a *.mrc file
into a CCP4.map file?
If not, is there any other way to do so?`
Alle the best,
Chris
DIPL.-ING. CHRIS FRÖHLICH
Crystallography Dept. | Daumke group
Max-Delbrück-Centre for Molecular Medicine (MDC)
Hi Ed,
1. Go to NCBI BLAST and run the sequence against the PDB subset. The
resulting list will have identities listed, so manual parsing is doable
if there aren't too many hits.
Have you looked at using NCBI EUtils for this? This might not solve
your problem directly, but it should
I don't know of anything directly within CCP4 - but mrc format is almost
identical to ccp4 map format. You could try using the mrc version as
ccp4 and see if it works. If not, upsalla mapman will convert brix to ccp4.
Pete
Froehlich, Chris wrote:
Dear colleagues,
is there any program
Froehlich, Chris chris.froehl...@mdc-berlin.de writes:
is there any program enclosed in CCP4 to convert a *.brix file or a
*.mrc file into a CCP4.map file?
If not, is there any other way to do so?`
MAPMAN from Uppsala (http://xray.bmc.uu.se/usf/) can read BRIX (and
write CCP4), not sure
Indeed, the intention is that .mrc and CCP4 .map are identical. There have been
some problems over the years, which I would call bugs, leading to apparent
incompatibility. I'd be interested in any specific cases of non-conforming
software (i.e. if there are some .mrc files which CCP4 programs
Dear CCP4 Community,
We are looking for a motivated Scientific Officer / Higher Scientific Officer
to join our exciting research programme on the regulation and functions of
ADP-ribosyltransferases. Please see the job description and instructions on how
to apply below.
Best wishes,
Sebastian
Hi,
We collected data using our in-house Bruker Proteum X8 diffractometer
and after using Xprep to integrate the images the output HKL file was
converted to an mtz using CCP4's Scalepack2mtz. The prp file generated
by Xprep shows good statistics with completeness and good data for 2.4
Hi Annette
Mosflm can only read Bruker images correctly after they have been converted
with frm2frm (available from Bruker) - this program unwarps them and writes
them in a format that Mosflm can understand.
On 25 Jun 2012, at 17:25, Annette Medina Morales wrote:
Also, I have recently tried
I'd like to alert subscribers to an exciting postdoctoral scientist
opportunity, using diffractive imaging to examine organelle-scale
biological structures at the Linac Coherent Light Source. The job posting
is listed at:
Dear Annette,
xprep (at least the xprep written by George Sheldrick, which comes with Bruker
machines) is not a data integration program!
Given that you collected data on a Bruker machine, presumably used saint/ the
apex software for data integration, then xprep, possibly after scaling the data
Dear Annette,
I suspect that a trivial error like confusing F and intensity is the
cause of your problem. You would probably get a better response if you
sent your message to the very informative Bruker mailing list or asked
one of the Bruker applications scientists. If you wish to send me
Are there any good antibodies for His-tags on the market. I have never used one
and I heard several stories that they were poor and not worth the money, over
the past few years. The only post I could find on the BB was from 2008. Have
His-Antibodies improved or are they still rubbish.
The one we have is rubbish. I can tell a vendor name if you want off-list.
A lot of non-specific bands + high background level makes it almost
impossible to use against cell lysate. It sort of works against purified
His-tag protein, but not perfect either.
On Mon, Jun 25, 2012 at 4:33 PM, D
We routinely use BSA-free anti-5His mAb from QIAGEN, and it works just fine (at
least using media and lysates of both insect and mammalian cells):
http://www.qiagen.com/products/protein/detection/qiaexpressdetectionsystem/penta-hisantibody.aspx
Good luck,
Luca
We also use that same antibody, and are satisfied overall.
On 6/25/12 8:59 PM, Luca Jovine wrote:
We routinely use BSA-free anti-5His mAb from QIAGEN, and it works just fine (at
least using media and lysates of both insect and mammalian cells):
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