Dear Qiangmin,
may be cleavage site is buried and not accessible for the protease? I am
not familiar with membrane proteins but you can consider this version.
Another question: is your protein N- or C- tagged?
08.04.2013 21:22, Qiangmin Zhang пишет:
Hello everybody,
I just purified a membrane
This paper compares the activity of various proteases (Prescission is GST
tagged HRV 3C) in detergents; PP should be active in DDM.
Vergis, J. M., Wiener, M. C. (2011). The variable detergent sensitivity of
proteases that are utilized for recombinant protein affinity tag removal.
Protein
Hello everybody,
i am a freshman in structural biology. Right now i encountered two questions
and need help from all of you:
1) from the paper, i saw there are three different interactions between the
ligand and the residue:(i) hydrogen bonding, (ii) π–π stacking and (iii)
cation–π
Hello everybody,
We have a diffraction data set from home source with 2theta at 20deg. But
we are not able to solve the data set using CCP4 suite. The software is
picking up only the lower resolution data but not the higher resolution
data points. Also we are not able to merge data two sets, one
Navin,
You mean that you can not PROCESS your data set? What diffraction data
processing program are you use?
What is your source, goniostat, diffractometer, area detector?
FF
Dr Felix Frolow
Professor of Structural Biology and Biotechnology, Department of Molecular
Microbiology and
Hi Navin
I'll assume you are using iMosflm from the CCP4 suite. Have you tried
increasing the resolution in the Processing Options window, under
the Processing tab?
The option to display resolution rings and change the resolution by
dragging the ring does not work at present in iMosflm.
Hello everybody,
We have a diffraction data set from home source with 2theta at 20deg. But
we are not able to solve the data set using CCP4 suite. The software is
picking up only the lower resolution data but not the higher resolution
data points. Also we are not able to merge data two sets, one
I thought imosflm recognizes automatically the 2theta offset ?
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry Molecular Biology
Johns Hopkins Malaria Research Institute
615 North Wolfe Street, W8708
Baltimore, MD 21205
Hello, Navin
Not enough information. What do you mean by picking up? Low I/s, high
Rmeas, too many alien spots? May be your crystals are twins with high
mosaicity, with ice rings and high background noise.
Please, share your processing log file (Mosflm?DENZO?)
Some home sources have additional
Dear Navin,
As others have pointed out, it is not clear where your problem exactly lies:
Are you not able to process the 2theta=20° run, or are you not able to merge it
with the 2theta=0° run? Anyways, I and others have had similar problems with
merging high and low resolution runs, see a very
hello everybody,
Thank you for all your suggestions. I am able to
process the 2 data sets separately. But the problem is that I dont have an
idea as to how can i merge the 2 data sets with different 2theta values.
On Tue, Apr 9, 2013 at 5:48 PM, herman.schreu...@sanofi.com
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Hello Navin,
what happens when you read them both into pointless, followed by
aimless? Once processed, the data should scale irrespective of the
2theta. If they don't they might be indexed differently, but pointless
would fix this.
Best,
Tim
On
Hello everybody,
I am working with a protein that should be covalently bound to an
organic ligand and I would calculate the correlation coefficient of this
ligand both against 2Fo-Fc and Fo-Fc map arising from refining (map
calculated in the presence of ligand).
To do this, I am using
A PhD fellowship is available in the laboratory of Dr. A. Dessen at the
Institut de Biologie Structurale in Grenoble, France, on structural biology
of proteins involved in the architecture of secretion systems of pathogenic
bacteria. The goal of this project is to crystallize, collect synchrotron
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