Re: [ccp4bb] is there any version of xia2 or XDSME compatible with new XDS?

2013-07-04 Thread Graeme Winter
Dear Rain Field, The current release is indeed unable to work with the newest versions of XDS and a new version should be available before the end of the week, which will be advertised on the blog. However this requires either a new nightly build of phenix or an unreleased cctbx build, as bits of

Re: [ccp4bb] is there any version of xia2 or XDSME compatible with new XDS?

2013-07-04 Thread Clemens Vonrhein
Dear all, sorry for the only half-related reply, but ... On Wed, Jul 03, 2013 at 09:20:56AM +0100, Rain Field wrote: Is there any similar software available? ... since you asked for similar software: the autoPROC release from 19th June is compatible. See

Re: [ccp4bb] Double stranded DNA concentration estimation

2013-07-04 Thread Opher Gileadi
Hi Appu, Adding the extinction coefficients of the nucleotides is a reasonable approximation; a somewhat more rigorous approximation takes into account nearest neighbor frequencies; see, for example, some commercial web sites such as

Re: [ccp4bb] PDB secondary structure assignments

2013-07-04 Thread MARTYN SYMMONS
Authors can supply the secondary structure assignments to the PDB and these trump the PDBs own determinations and will appear in the authors' entry. Remarks 650 (Helix) and 700 (Sheet) are then included which allow the documentation of the origins of the secondary structure definitions and any

[ccp4bb] Call for access to Synchrotron Beamline Facilities, autumn 2013 - EMBL, Hamburg, Germany

2013-07-04 Thread margret
Call for access to Synchrotron Beamline Facilities, autumn 2013 - EMBL, Hamburg, Germany As you may already be aware, due to unforeseen circumstances related to the civil construction of the PETRA III extension, DESY has postponed the PETRA III shutdown and user operation will continue until

Re: [ccp4bb] Split Crystal Dataprocessing

2013-07-04 Thread RHYS GRINTER
Hi All, Thanks for all your responses and help. I'll be trying all of these things over the coming couple of weeks. Rhys From: pshawstew...@gmail.com [pshawstew...@gmail.com] On Behalf Of Patrick Shaw Stewart [patr...@douglas.co.uk] Sent: 04 July 2013

[ccp4bb] XDS not picking spots correctly, please help!

2013-07-04 Thread Fei Li
Dear all, I recently collected some 10 degree wedges of data on a very small membrane protein. The data processing so far is not working well. XDS is the only software that process my data and give a space group and cell parameters. However, I ended up with a merged dataset with low

[ccp4bb] Stereo images

2013-07-04 Thread David Schuller
http://io9.com/rare-3d-camera-found-containing-photos-from-wwi-669397198 Rare 3D Camera Found Containing Photos from WWI http://io9.com/rare-3d-camera-found-containing-photos-from-wwi-669397198 -- === All Things Serve the

Re: [ccp4bb] XDS not picking spots correctly, please help!

2013-07-04 Thread Bosch, Juergen
Hi Fei, I assume you have edited the XDS.INP file to include the initially found space group and the first refined cell ? If not, then that would be my first choice to do. If that then still fails with the default parameters, then I would start fixing some values instead of refining all of

Re: [ccp4bb] XDS not picking spots correctly, please help!

2013-07-04 Thread Fulvio Saccoccia
Dear Fei, in addition, take a look here: https://www.researchgate.net/profile/Fulvio_Saccoccia/contributions/?ev=prf_act and download my tutorial on XDS if you want. Bye Il 04/07/2013 18:26, Fei Li ha scritto: Dear all, I recently collected some 10 degree wedges of data on a very

Re: [ccp4bb] is there any version of xia2 or XDSME compatible with new XDS?

2013-07-04 Thread Rain Field
XDSme works fine right now. Thank you all!

Re: [ccp4bb] Double stranded DNA concentration estimation

2013-07-04 Thread Ho Leung Ng
Hello Appu, Small amounts of strongly UV absorbing contaminants can throw off your measurements. If you want to be very accurate, try a fluorescent dye specific for double stranded DNA such as PicoGreen. Ho Ho Leung Ng University of Hawaii at Manoa Assistant Professor, Department of