Dear All
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Hi,
what was not mentioned in the ccp4 discussion is
The use of trimethylamine N-oxide as a primary
precipitating agent and related methylamine
osmolytes as cryoprotective agents for
macromolecular crystallography
Acta Cryst. (2012). D68, 69-81
I found it as a very potent compound for
Jacob is right, there definitely seem to be problems with the data. Perhaps you and your supervisor should consider contacting privately the developers of data processing programs
that have participated in the thread like Kay and Harry (and others perhaps too) to try and get the best out of
(Off-list)
Dear Kay and Harry,
You mentioned difficult datasets being of interest for the ACA conference. I am
not coming, but do have some interesting datasets, viz., many datasets of a
particular crystal form of a calmodulin/peptide complex which have defied my
[not exhaustive] attempts to
Hi Vicky,
I tried to change the ratio of paraffin:silicon oil to 1:2, but still the
protein precipitated. So I tried to do it other way round, I kept
paraffin:silicon oil ratio as 2:1 and so far it has not precipitated. Apart
from that, I separately used the two oils.In silicon oil there was
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Dear Prerana,
is there a reason you do not try vapour diffusion instead of batch
crystallisation? You can have better control about the rate of change
which might affect the behaviour of the sample.
Regards,
Tim
On 02/24/2014 05:23 PM, Prerana G.
Dear BB-lers,
I want to draw in pymol 2 objects, which are behind each other, as
transparent surfaces.
The surface at the back gets simply deleted. This would be correct (save
some rendering time) if the object at the front was not see-thru.
What wheel do I need to turn to get the surface of
Hi Matthias,
you can switch to multi-layer transparency:
PyMOL set transparency_mode, 1
This works for ray-tracing. In real-time (OpenGL) rendering, it currently
depends on which object was enabled first. So try to toggle the back object off
and on in the object menu panel.
Cheers,
Thomas
