[ccp4bb] Serial Crystallography Workshop

2023-06-27 Thread Jeney Wierman
Hello all, We are in the midst of organizing a two-part workshop focusing on developments in serial crystallography, specifically addressing time-resolved approaches and reliable and routine methods for user operation. We invite you to participate in the open forum portion of the workshop,

[ccp4bb] CryoEM Current Practices Webinar This Thursday, 06/29/2023

2023-06-27 Thread Christina Zimanyi
Dear all, Please join the NIH Transformative High Resolution CryoEM Program service centers this week for our CryoEM Current Practices webinar. Time: Thursday, June 29, 2023 at 9 AM pacific / 12 PM eastern time Speaker: Shubham Dubey & Michelle Bush, Graduate Students, Noinaj Lab, Department of

Re: [ccp4bb] Translational NCS (tNCS)

2023-06-27 Thread Eleanor Dodson
WELL - with such high LLG and translation NCSthe high R factors are a bit surprising.. The Patterson info does not give the relative height of the origin peak to the off origin one.. Questions which I would check. Look at the data processing carefully. Are there bad batches? Could the space group

[ccp4bb] CCP4 Cloud workshop IGBMC, Illkirch, France

2023-06-27 Thread Maria Fando - STFC UKRI
WORKSHOP CCP4 Cloud for structure solution Tuesday 26 September 2023 Auditorium, IGBMC Maria Fando and Eugene Krissinel CCP4, UKRI-STFC, Rutherford Appleton Laboratory, Didcot, United Kingdom The workshop will begin with a lecture overview of the development of structural solution

[ccp4bb] Postdoctoral position in method development (York Structural Biology Laboratory)

2023-06-27 Thread Jon Agirre
Dear all, Applications are invited for a Postdoctoral Research Associate position, funded by the Royal Society, for up to 30 months to work with Dr Jon Agirre in the Department of Chemistry. The successful applicant will join a vibrant team of researchers at the heart of the world-leading York

[ccp4bb] Need suggestion

2023-06-27 Thread Afshan Begum
Dear expert, I am currently working on building my cryo-EM data using COOT. However, I have encountered an issue when trying to add monomers such as Glycerol or BCT using their letter code from the "Get monomer" option in COOT. Whenever I attempt to merge them into my coordinate, COOT crashes. 

Re: [ccp4bb] Script to refine water molecules - help

2023-06-27 Thread Paul Emsley
The Coot API is quite useful for this sort of thing imol = 0 # replace as needed residues = residues_matching_criteria(imol, lambda chain_id, res_no, ins_code, serial: residue_name(imol, chain_id, res_no, ins_code) == "HOH") refine_residues(0, residues) Regards, Paul. On 26/06/2023

Re: [ccp4bb] Need suggestion

2023-06-27 Thread Paul Emsley
On 27/06/2023 15:01, Afshan Begum wrote: Dear expert, I am currently working on building my cryo-EM data using COOT. However, I have encountered an issue when trying to add monomers such as Glycerol or BCT using their letter code from the "Get monomer" option in COOT. Whenever I attempt to

Re: [ccp4bb] Translational NCS (tNCS)

2023-06-27 Thread Esko Oksanen
Hi Bashir, It does look like you have translational pseudo-symmetry. If the translation vector happens to be body-centering, it could affect your intensity statistics so that the R-values would never go down as half of your reflections follow the distribution for centric reflections. I had