Dear all,
Anyone who have had success using the mosquito for micromatrix seeding?
Which way is the optimal way of adding seed solution to sitting drops
on an MRC plate?
Is it better to add 100 nl protein + 10 nl seed solution + 100 nl
reservoir solution or
to add concentrated seed solution
Hi Ian,
Am 19.09.10 15:25, schrieb Ian Tickle:
Hi Florian,
Tight NCS restraints or NCS constraints (they are essentially the same
thing in effect if not in implementation) both reduce the effective
parameter count on a 1-for-1 basis.
Restraints should not be considered as being added to the
Dear Maria
In our hands Mosquito MMS worked by adding 2x protein solution + 1x seed
solution + 1x reservoir. The reason for doing this was two-fold:
I) ratio protein/reservoir is similar to the condition that generated the seed
stock
II) a deliberate bias toward the initial condition is given
Dear all,
I wanted to generate the 301'st project in the CCP4i interface
Directories+Projects. The problem is that when I click on the button for
the Project of this session, there is apparently no space for the 13th
column (of 25 entries). I do not want to delete any old projects, so is
there
dear Maria,
we routinely use Mosquito for MMS, the ratio works the best: 100nl protein +
multi-aspirate mode x (15nl seeds + 85nl reservoir solution).
best regards,
alexey
Alexey RAK
Structural Biology
SDI/LGCR
Sanofi-aventis RD
Centre de Recherche Paris
13, Quai Jules Guesde - BP 14
Hi Dirk
First, constraints are just a special case of restraints in the limit
of infinite weights, in fact one way of getting constraints is simply
to use restraints with very large weights (though not too large that
you get rounding problems). These 'pseudo-constraints' will be
indistinguishable
Eric,
if you want to use stereo on a notebook you have very limited options. I have
stereo running using a Lenovo T61p with a Nvidia Quadro FX570M under XP, not
tried Linux yet. Officially this card is not even supported by Nvidia for 3D
application, but works anyway in conjunction with a
The Quadro FX 3700M in my Dell Precision 6400M notebook works great with
stereo in Windows, no support for Linux yet though.
On Mon, Sep 20, 2010 at 8:57 AM, Schubert, Carsten [PRDUS]
cschu...@its.jnj.com wrote:
Eric,
if you want to use stereo on a notebook you have very limited options. I
Hi Nicholas,
Thank you for your reply.
snip
it seems that we are trying to deposit one model to satisfy two
different purposes - one for model validation and the other for model
interpretation (use in docking etc), and what's good for one purpose
might not be necessarily good for the other.
Dear colleagues,
The EBI is carrying out a user survey about its services, collecting input
from current and potential users. The survey lives here:
http://www.surveymonkey.com/s/EMBL-EBI
- It should take 10-15 minutes.
- There are no compulsory questions.
- You can be
It's best to have dedicated NVIDIA (don't have much experience with ATI,
but it is my understanding that they may be more difficult to configire
sometimes). However, Intel on-board graphics has gotten much better
recently (in fact, Intel releases drivers as open source (guess because
they are not
Hi Eric,
I wanted to know which type of graphics card is more suitable for a notebook
which is going to be used for structural biology. Integrated or dedicated?
ATI or NVIDIA? At the moment I have to choose between an integrated Intel HD
Graphics or a dedicated NVIDIA NVS 3100M Graphics.
A postdoctoral position in protein crystallography is available in Dr. Min Lu’s
lab at Public Health Research Institute Center, New Jersey Medical School,
University of Medicine and Dentistry of New Jersey.
Our research mainly focuses on the three-dimensional structures and biological
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