Dear Theresa
Cryo-cooling the crystals straight out of their drops or after brief
incubations in crystal stabilization solutions containing 2M ammonium sulfate
may be the way to go.
Here is a copy/paste piece from Kyndt et al (2007) Biochemistry 46, 95-105.
All the best
Savvas
Aliquots (0.5 í
Dear all,
Thanks a lot for good suggestions on this topic. To answer some of the
questions: The apo strucuture has not been solved; the crystals dissolved upon
soaking (my term 'crack' was not accurate); and I have not tried
co-crystallization.
A brief summerization here:
1)
Computational Systems Biology Postdoctoral Position in Translational
Computational Systems Biology
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to work with Professor Jeffrey Skolnick at the Georgia Tech Center for the
Study of Systems Biology in the following
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Postdoctoral applicants are sought in Computational Systems Biology to work
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application of novel algorithms for the:
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Dear All
This is rather off topic for the CCP4 BB but I would like to draw people's
attention to developments currently taking place at diamond.
We require a postdoctoral scientist to join an exciting and innovative project
to develop methods associated with the soft X-ray imaging of
A postdoctoral position is available in the Laboratory of Molecular
Mechanisms of Membrane Transport at the Institut Pasteur in Paris (France)
to study excitatory neurotransmitter transporters. Our work focuses on the
structural, energetic and dynamic components of the function of these
membrane
Aline does also read a dssp file and print the schematic representation above.
Probably this is also possible with STRAP. I am not sure which program exactly
the pdb use for their diagrams.
Regards
Christian
Am Samstag 04 Februar 2012 12:11:09 schrieb Vellieux Frederic:
I believe Procheck
On Sun, 2012-02-05 at 22:49 +, Theresa H. Hsu wrote:
Crystals are from 2 M ammonium sulfate.
begin \personal_bias
Sodium malonate is your friend
http://scripts.iucr.org/cgi-bin/paper?fw5004
end
--
Oh, suddenly throwing a giraffe into a volcano to make water is crazy?
Hello,
here is a question about the EXPAND command in SFTOOLS, specifically
its effect on a free reflection flag. Do the flag values get copied to
newly generated reflections based on symmetry, for example in the case
of a P622 - P6 expansion?
many thanks,
Wolfram Tempel
On 12-02-06 08:37 AM, wtempel wrote:
Hello,
here is a question about the EXPAND command in SFTOOLS, specifically
its effect on a free reflection flag. Do the flag values get copied to
newly generated reflections based on symmetry, for example in the case
of a P622 - P6 expansion?
many thanks,
I just installed ccp4 6.2.0 on a new RHEL6.2 system and I get this error.
Anyone know how to fix this? I installed tcl and tk into the system but ccp4
seems to
want to use its own version.
[xray@paprika ~/Desktop]$ /home/xray/ccp4/ccp4-6.2.0/ccp4-6.2.0/bin/ccp4i:
When using oil on protein crystal mounts I suggest:
1. once the crystal is under oil, remove as much adhered solution from the
crystal surface.
2. if there are any volatile components in your drop condition, presaturate
the
oil with that solvent.
3. consider using perfluoropolyether since
Theresa,
Several suggestions that have been given are excellent advice:
Li salts suggested by Tommi Kajander is what I would use
in particular:
80% saturated lithium sulfate.
This should work. I would be very surprised if it does not.
Malonate as suggested by Sean Seaver is another great idea,
6-Feb-2012
Dear Marilyn
Have you looked at Proteopedia
[www.proteopedia.orghttp://www.proteopedia.org], see e.g.
http://proteopedia.org/w/DNA
http://proteopedia.org/w/Forms_of_DNA
http://www.proteopedia.org/w/HIV-1_protease
http://www.proteopedia.org/w/Proton_Channels
and many more.
n.b. How the
-BEGIN PGP SIGNED MESSAGE-
Hash: SHA1
Dear kas,
The error message (bad ELF interpreter) suggests a mismatch between a
64bit library and a 32bit binary (or vice versa), i.e. your system may
lack compatibility libraries to run 32bit programs. These are usually
available for linux
Hello
If I am not wrong, there was one posting related with SASX workshop where we
can also bring own samples. Does someone have that information? Please send me
the link.
Thanks a lot
Amit
http://www.embl-hamburg.de/training/events/2012/SAXS/
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Luthra,Amit
Sent: 06 February 2012 17:27
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] SAXS workshop
Hello
If I am not wrong, there was one
Your 64bit system does not have the 32bit LSB libraries installed. Install the
packages redhat-lsb.i686 and redhat-lsb-graphics.i686 using yum as follows:
yum install redhat-lsb.i686 redhat-lsb-graphics.i686
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On
Dear Theresa,
Gary Gilliland's paper on cryosalts would seem to be useful for your problem.
http://scripts.iucr.org/cgi-bin/paper?en0028
Also, I have used 15% glycerol in a synthetic mother liquor to effectively
freeze crystals grown in 2M Ammonium Sulfate. Another method that Jim Pflugrath
On 02/06/12 12:26, Luthra,Amit wrote:
Hello
If I am not wrong, there was one posting related with SASX workshop where we
can also bring own samples. Does someone have that information? Please send me
the link.
Thanks a lot
Amit
http://www.chess.cornell.edu/BioSAXS%20course/index.htm
And here's one from Brookhaven -- app. deadline is three weeks away:
http://workshops.ps.bnl.gov/default.aspx?w=SAXSMar2012
http://www.chess.cornell.edu/BioSAXS%20course/index.htm
Getting Started in Biological Small-Angle X-ray Solution Scattering
Feb 24-26, 2012
Students will
All,
We have a summer internship opening at Merck (see below for the details)
for graduate students in the Global Structural Chemistry/X-ray
crystallography/Biomolecular NMR group at either its Kenilworth, NJ or West
Point, PA sites. Internships will begin in early June 2012, and will run
Hi all
Thanks for all the suggestions which I will try soon.
How do the crystallization condition (PEG vs. salts like ammonium sulfate)
affect the croyprotectant condition? Do factors like presence of low
concentration of high molecular weight PEG ( 2000) mean PEG is better? Do
buffers and
Dear colleagues,
As many of you know, the CASP community experiments have been running
once every two years since 1994, collecting information on soon to be
solved structures from the experimental community, and passing on
sequence data to the structure modeling community so that blind
Dear All
For calculation of the shape complementarity index using the Sc
program between a protein and DNA surface, does anybody have a
modified radii.lib file that includes information about DNA atoms?
Also, is there any reference that has information regarding the atomic
radii of DNA atoms?
Hi Theresa,
What works well for me, is to use like with like, e.g. to use salts or
salt-like cryoprotectants for salt conditions (e.g. malonate,
li-sulfate, sucrose/xylitol for ammonium sulfate conditions) and
glycerol, low mw PEG etc. for PEG and other alcohol conditions. I have
very bad
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