Dear CCP4BB,
I have a question regarding sending protein crystals at ~21 deg C. I was
advised to keep the capillaries containing the crystals in a thermos
layered with a few bags of heptadecane (mp 20-22 C). I have been searching
for quite some time about the working concentration of heptadecane f
On Thu, Jun 6, 2013 at 2:37 PM, GRANT MILLS wrote:
> My script seems to miscount the columns and read the two as one column,
> does anyone know how to avoid this? (PS, I've googled this like crazy but I
> either don't understand or the link is irrelevant)
>
You should resist the temptation to w
Dear CCP4BB,
I'm trying to write a simple python script to retrieve and manipulate PDB data
using the following code:
#for line in open("PDBfile.pdb"):
#if "ATOM" in line:
#column=line.split()
#c4=column[4]
and then writing to a new document with:
#with open("selection.pdb"
Hi Yuri,
http://fpocket.sourceforge.net
http://sts-fw.bioengr.uic.edu/castp/calculation.php
Just to name 2
Jürgen
On Jun 5, 2013, at 7:12 PM, Yuri Pompeu wrote:
> Dear BB,
> I am sorry for posting off-topic but it is hard not to ask when you know you
> can get a good answer ;-)
>
> I need to
Dear BB,
I am sorry for posting off-topic but it is hard not to ask when you know you
can get a good answer ;-)
I need to calculate the volume of several active sites. Nothing fancy, just a
number for comparison sake.
I understand there are probably 10 different ways/programs and I would
apprec
Dear Olga,
I always run SHELXC/D/E from hkl2map because of the nice graphical
diagnostics while the programs are running.
If you start hkl2map, give any project name and choose 'more options'
you will be able to specify that you need more memory.
From your comment I suspect that you are using
Dear All,
I am trying to run shelx c/d/e from ccp4 interface (latest updated version of
CCP4). The program terminates with message:
"Arrays too small to generate reflection data -try -L1". (Which, as I
understand, means I have too many reflections, and that one way around the
problem
is to use
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Thermo Scientific/Pierce has gotten into the protein purification game
recently. The resin is slightly cheaper (USD $975/100 ml versus $1656 for
GE) and the binding capacity is equivalent. I haven't rigorously run any
regeneration tests though. Our local sales rep actually sent us a 1 ml spin
colum
Hi Joern,
have a look at these two papers (and probably KK is reading this too and can
chime in with the right set of coordinates),
Korotkov et al. Secretins: dynamic channels for protein transport across
membranes. Trends Biochem Sci (2011) vol. 36 (8) pp. 433-43
Reichow et al. Structure of
Dear Tim,
the rings I mean are similar to the one in 4FC4 but to my understanding
FocA does not form helices under physiological conditions.
I am sorry if I was not specific enough. I will give you more details. I
am working on two proteins which are dimers in solution.
The first of them crysta
I too am not sure what is being asked but do the TMV helix and disk structures
count? See for example slide 3 in
http://users.ipfw.edu/blumenth/Virology/Assembly.PDF
Colin
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Tim Gruene
Sent: 05 June 2
-BEGIN PGP SIGNED MESSAGE-
Hash: SHA1
Dear Joern,
I am not sure this is what you have in mind: just yesterday I heard a
talk from Oliver Einsle about FocA ion channels - look at PDB ID 4FC4,
for example forming a (flat) pentamer, and let us know if this is what
you mean.
Best,
Tim
On 06
Hi all.
I don't have any experience with Clontech and Fisher
resins, but about the GE one I faced the same problem as
Sebastiano indicated.
But I have to say that the problem was not general, but
protein (or family of protein) related: the low binding
depends on the oligomerization state of t
Please direct your inquiries to Dr Garcia:
Dear Colleagues, I have an opening for a senior post-doc or staff level
crystallographer in my group. This is an ideal transitional position for
someone at later stages of a post-doc who is looking to bolster
experience and/or publication record
Hi Mirek, hi all,
I'm also very interested in the topic, so please keep me up with the replies,
or make sure to post a summary, please.
In addition to the price, the problem we're facing with GSH-beads from GE
(although we haven't tried others yet) is that we can't manage to deplete our
lysate
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