Thanks for this other suggestions similar (see below.)
My problem is not the distances per se, these restrain fine to 2.3A
I am relatively low resolution and my problem is with just distance restraints
I move to a square planar arrangement. I suspect this is a feature of the
geometry
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Dear James,
I am not sure I fully picture what your coordination looks like, but my
guess is that if you include distance restraints between the S-atoms
you can distinguish between a tetrahedral geometry and a square pyramid.
You could use external
Dear ccp4bb users,
I have a data set collected at 2.2A resolution that I can integrate
in P4. Aimless, Phaser and Xtriage recognized a pseudotranslation
(34.6% of origin peak) at fractional coordinates 0.500 0.500 0.479
(ORTH 46.7 46.7 37.4). However labelit.index run with
*
11th International Conference: Biology and Synchrotron Radiation (BSR),
8-11 September 2013, EMBL Hamburg, Germany *
The abstract submission deadline for BSR-2013 is *extended* until
*Thursday 20th June*, 2013!
Biologists, physicists, chemists, from students to principal
investigators,
Andrew Leslie made a similar suggestion for using CB to S distances.
Both make me unhappy for the reason that there is not a clear chemical basis to
them (Tim's has more a chemical basis) and how would I choose the distance. (I
can measure it from a known structure)
I have previously
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Hi Jim,
the chemical basis would be that the Zn connects to all four S atoms,
and if they are indistinguishable, all Zn-S distances should be alike.
But the S-atoms are not bonded to each other, hence they want to have as
much distance from each
Dear CCP4BB users,
I've recently joined TTP Labtech with the responsibility of all mosquito
related sales into the United Kingdom and Europe. I'm keen to find out how
many mosquito users we have in this forum and what applications you're
currently using the instrument for. The non-mosquito
Well, when I solved a structure for a protein-rhenium adduct I had to
manually code a restraint file for the rhenium tricarbonyl fragment,
which is octahedral overall (with proteins ligands and waters), and had
several different types of bond distances for the various ligands. I
started with
Is there a reason why you must use restraints with metal coordination?
The main reasons that you use restraints in protein refinement is to
reduce the number of free variables in the refinement, and because the
resolution isn't sufficient to resolve two atoms separated by the
typical
Please find the link to the advertised BLS position
http://www.psi.ch/pa/offenestellen/0601-1.
Beamline Scientist
Protein Crystallography Beamlines at SLS
Your tasks
As a beamline scientist you will join a team effort to operate, maintain and
develop protein crystallography beamlines at the
Nat,
I agree that deep understanding of column-formatted text isn't really
necessary (there's not much internal complexity there).
I was attempting to point out that the general rule of not re-inventing
the wheel doesn't always apply. Even when we're addressing biological
questions, if
Hi Pete,
it's surely beneficial and good to know how software works especially if
it's part of your research, I entirely agree. Though, on the other hand, I
don't feel too bad about not knowing the CS behind the implementation of MS
Word when I use it for my documents editing. I guess it depends
Lawrence Berkeley Laboratory
Postdoctoral Researcher
Job ID: 76029
Division: Physical Biosciences (Joint BioEnergy Institute)
Date Opened: 6/5/2013
Summary: A postdoctoral position is available at the Joint BioEnergy Institute
in Emeryville to work on proteins involved in plant cell wall
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