[ccp4bb] substructure solution.

2015-02-23 Thread Avisek Mondal
Dear all, I am trying to phase a large novel structure of 150 kDa with P21 space group. So far I have collected 3.0A datasets from iodinated derivatives in Cu-K alpha. In SAD phasing I got 0.745 figure of merit (FOM) and LLG about 476208.27 (sites in inverse hand gave the same FOM and LLG),by

[ccp4bb] New: PDB_REDO version 6 (now also for OSX)

2015-02-23 Thread Robbie Joosten
Dear CCP4BB subscribers, We are happy to announce the release of PDB_REDO version 6. PDB_REDO is a pipeline for crystallographic structure model optimisation, and you can use it through: 1. The PDB_REDO webserver at http://xtal.nki.nl/PDB_REDO 2. Local installations available for Linux

[ccp4bb] Postdoctoral position in Biochemistry/Molecular Biology, Stockholm University

2015-02-23 Thread Matthew Bennett
Postdoctoralpositionin Biochemistry/Molecular Biology ”Enzyme evolution, withribonucleotide reductases as model system” at Department of Biochemistryand Biophysics, Stockholm University Application deadline:

Re: [ccp4bb] Blob Game

2015-02-23 Thread Keller, Jacob
...And the cryoprotectant was simply the Li/NH4SO4 mother liquor. Also maybe relevant: the crystals were pretty old, a year or so. JPK -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Keller, Jacob Sent: Monday, February 23, 2015 12:21 PM To:

[ccp4bb] Position available at Molecular Dimensions- must love all things crystallography

2015-02-23 Thread Jeanette Hobbs
Don't know anything about sales or marketing but know a lot about structural biology and all things crystallography and love talking to people about it?...then this job is for you. We have a position open for a European Technical Sales Specialist Could you develop our sales in Europe by

[ccp4bb] CCP4-6.5 Update 004

2015-02-23 Thread Charles Ballard
Dear CCP4 Users An update for the CCP4-6.5 series has just been released, consisting of the following changes * ccp4 updater (all platforms) - allows for offline updates - details at http://www.ccp4.ac.uk/updates * pointless (all) - update to 1.9.27 - update for processing XDS INTEGRATE files

Re: [ccp4bb] Blob Game

2015-02-23 Thread Keller, Jacob
Ethanol? You weren't setting up drops with liquor on your breath, were you? I can't remember. JPK On 02/23/2015 12:20 PM, Keller, Jacob wrote: Dear Crystallographers, I've got a strange blob hanging off an arginine--see attached. Any ideas? Nothing weird in the prep; cryst+prot conditions:

Re: [ccp4bb] Blob Game

2015-02-23 Thread jens Preben Morth
a horrible water molecule would fit in there :-) On 23/02/15 19:42, Keller, Jacob wrote: A little more info: -Resolution is 1.75 Ang at edge of detector, but I/sig was still ~3; pushing integration to corners gives 1.66 Ang, but completeness is horrible. The home setup was at the limit

Re: [ccp4bb] Blob Game

2015-02-23 Thread Keller, Jacob
A little more info: -Resolution is 1.75 Ang at edge of detector, but I/sig was still ~3; pushing integration to corners gives 1.66 Ang, but completeness is horrible. The home setup was at the limit (without 2 theta.) -Data are horribly twinned with fractions of 58/42%, spacegroup is P3212.

Re: [ccp4bb] Blob Game

2015-02-23 Thread Edward A. Berry
Ethanol? You weren't setting up drops with liquor on your breath, were you? On 02/23/2015 12:20 PM, Keller, Jacob wrote: Dear Crystallographers, I've got a strange blob hanging off an arginine--see attached. Any ideas? Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES,

Re: [ccp4bb] Blob Game

2015-02-23 Thread Boaz Shaanan
Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel E-mail: bshaa...@bgu.ac.il Phone: 972-8-647-2220 Skype: boaz.shaanan Fax: 972-8-647-2992 or 972-8-646-1710 From: CCP4 bulletin board

Re: [ccp4bb] Blob Game

2015-02-23 Thread Keller, Jacob
Just a little mother liquor. I am seeing what SO4 does in refinement right now. JPK -Original Message- From: Boaz Shaanan [mailto:bshaa...@bgu.ac.il] Sent: Monday, February 23, 2015 2:50 PM To: Keller, Jacob; CCP4BB@JISCMAIL.AC.UK Subject: RE: [ccp4bb] Blob Game Boaz Shaanan, Ph.D.

Re: [ccp4bb] Blob Game

2015-02-23 Thread Oliver Smart
on 23/2/15 6:57 PM, jens Preben Morth j.p.mo...@ncmm.uio.no wrote: a horrible water molecule would fit in there :-) On 23/02/15 19:42, Keller, Jacob wrote: A little more info: -Resolution is 1.75 Ang at edge of detector, but I/sig was still ~3; pushing integration to corners gives 1.66

[ccp4bb] Open PhD and Postdoc positions in Bioinformatics

2015-02-23 Thread Mario Albrecht
Dear colleagues, Please forward these bioinformatics job offers for PhD students and Postdocs to others, thank you much! The positions are relevant for structural bioinformatics. Kind regards, Mario Albrecht Open PhD and Postdoc positions in

[ccp4bb] Postdoc Position in NanoMEGAS SPRL, Belgium

2015-02-23 Thread Dr. Partha Pratim Das
Dear Colleagues, Postdoctoral associate in NanoMEGAS SPRL, Belgium Description: We are looking for an outstanding and highly motivated individual to start immediately in a European funded postdoctoral position based in Brussels (www.nanomegas.com) on analysis of nanomaterials by Electron and