Dear all,
I am trying to phase a large novel structure of 150 kDa with P21 space
group. So far I have collected 3.0A datasets from iodinated derivatives in
Cu-K alpha. In SAD phasing I got 0.745 figure of merit (FOM) and LLG about
476208.27 (sites in inverse hand gave the same FOM and LLG),by
Dear CCP4BB subscribers,
We are happy to announce the release of PDB_REDO version 6.
PDB_REDO is a pipeline for crystallographic structure model optimisation,
and you can use it through:
1. The PDB_REDO webserver at http://xtal.nki.nl/PDB_REDO
2. Local installations available for Linux
Postdoctoralpositionin Biochemistry/Molecular Biology
”Enzyme evolution, withribonucleotide reductases as model
system” at Department of Biochemistryand Biophysics,
Stockholm University
Application deadline:
...And the cryoprotectant was simply the Li/NH4SO4 mother liquor. Also maybe
relevant: the crystals were pretty old, a year or so.
JPK
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Keller,
Jacob
Sent: Monday, February 23, 2015 12:21 PM
To:
Don't know anything about sales or marketing but know a lot about structural
biology and all things crystallography and love talking to people about
it?...then this job is for you.
We have a position open for a European Technical Sales Specialist
Could you develop our sales in Europe by
Dear CCP4 Users
An update for the CCP4-6.5 series has just been released, consisting
of the following changes
* ccp4 updater (all platforms)
- allows for offline updates
- details at http://www.ccp4.ac.uk/updates
* pointless (all)
- update to 1.9.27
- update for processing XDS INTEGRATE files
Ethanol? You weren't setting up drops with liquor on your breath, were you?
I can't remember.
JPK
On 02/23/2015 12:20 PM, Keller, Jacob wrote:
Dear Crystallographers,
I've got a strange blob hanging off an arginine--see attached. Any ideas?
Nothing weird in the prep; cryst+prot conditions:
a horrible water molecule would fit in there :-)
On 23/02/15 19:42, Keller, Jacob wrote:
A little more info:
-Resolution is 1.75 Ang at edge of detector, but I/sig was still ~3; pushing
integration to corners gives 1.66 Ang, but completeness is horrible. The home
setup was at the limit
A little more info:
-Resolution is 1.75 Ang at edge of detector, but I/sig was still ~3; pushing
integration to corners gives 1.66 Ang, but completeness is horrible. The home
setup was at the limit (without 2 theta.)
-Data are horribly twinned with fractions of 58/42%, spacegroup is P3212.
Ethanol? You weren't setting up drops with liquor on your breath, were you?
On 02/23/2015 12:20 PM, Keller, Jacob wrote:
Dear Crystallographers,
I've got a strange blob hanging off an arginine--see attached. Any ideas?
Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES,
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben-Gurion University of the Negev
Beer-Sheva 84105
Israel
E-mail: bshaa...@bgu.ac.il
Phone: 972-8-647-2220 Skype: boaz.shaanan
Fax: 972-8-647-2992 or 972-8-646-1710
From: CCP4 bulletin board
Just a little mother liquor. I am seeing what SO4 does in refinement right now.
JPK
-Original Message-
From: Boaz Shaanan [mailto:bshaa...@bgu.ac.il]
Sent: Monday, February 23, 2015 2:50 PM
To: Keller, Jacob; CCP4BB@JISCMAIL.AC.UK
Subject: RE: [ccp4bb] Blob Game
Boaz Shaanan, Ph.D.
on 23/2/15 6:57 PM, jens Preben Morth j.p.mo...@ncmm.uio.no wrote:
a horrible water molecule would fit in there :-)
On 23/02/15 19:42, Keller, Jacob wrote:
A little more info:
-Resolution is 1.75 Ang at edge of detector, but I/sig was still ~3;
pushing integration to corners gives 1.66
Dear colleagues,
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Open PhD and Postdoc positions in
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