Hi Didi,
Does the PDB file have unique chain identifiers, or is this a concatenation of
multiple chains which us the same identifier? If that's the case, try this:
PyMOL> set retain_order
See also:
https://pymolwiki.org/index.php/retain_order
Hope that helps.
Cheers,
Thomas
On 11 Nov 2016,
Hi all,
I want to use PyMOL to draw an image of a big protein complex with a virus
capsid-like protein shell and an enclosed enzyme. But it only works in line
mode. If I show it in cartoon mode, most residues went missing. Did anyone come
across the same problem and could you give me some sugge
Once again, chemical intuition may help. At neutral pH values, sulfate
is going to be present at SO4(2-), whereas phosphate will be present as
H2PO4(-) or HPO4(2-). The hydrogen bond network supporting binding may
be able to offer clues. Sulfate is not likely to have any H-bond
acceptors in its
Assuming it wasn't clear from purification/crystallisation reagents...
Maybe try a high multiplicity anomalous dataset collected in house / at
long wavelength?
P has ~ 75% the f" of S at CuKa.
If you can figure out roughly what anomalous peak height an S atom gives
from a Cys or a Met with simil
Similarly, how do you differentiate a phosphate ion than sulfate just based
on electron density if data is not at atomic resolution?
Thanks!
On Fri, Nov 11, 2016 at 3:52 AM, Harry Powell
wrote:
> Hi all
>
> Sticking to the first question, if you don't restrict yourself to _X-ray_
> crystallogr
Hi Ian,
I'm aware that this can be done with Buster (and probably also
> phenix.refine): I'm asking specifically about Refmac.
>
since you mentioned phenix.refine:I confirm it is possible to freeze any
refinable parameters (coordinates, occupancy, xyz and iso/anisotropic B
factors for any selecte
Good morning all
Just to follow up on the list, at Instruct we publish opening for structural
biologists
around the world.
https://www.structuralbiology.eu/update/jobs/
Best wishes
Claudia
Dr Claudia Alen Amaro
Scientific Project Manager
Instruct: An Integrated Structural Biology Infrastruct
Hi all
Sticking to the first question, if you don't restrict yourself to _X-ray_
crystallography but use your local neutron source instead, it should be
straightforward (subject to all the normal caveats).
On 10 Nov 2016, at 23:02, Tim Gruene wrote:
> Dear JPK,
>
> to answer your first questi
Hi
I'd ask on a small molecule forum (e.g. in UK try
XRAY Forum - http://www.xrayforum.co.uk/
) or check some of the (inter)national associations' websites, e.g.
IUCr - http://www.iucr.org/people/employment
ECA - http://ecanews.org/mwp/jobs-and-positions/
BCA
