Job opening for a structural biologist to join the Structural Motility team at
the Curie Institute Paris, France.
We are looking for a post-doctoral fellow to join the team Structural Motility
at the Curie Institute (Paris Center) directed by Anne Houdusse.
The Structural Motility team at the
Folks
Calling for articles and short communications of interest to
structural biologists. The deadline for submission for the January
202-0 issue is 15 November 2019.
The Computational Crystallography Newsletter (CCN) is an electronic
newsletter for structural biologists, and is published online
Hi everyone,
thx for the overwhelming response,
it looks like some of us experienced the very same problem,
in a nutshell, dependent on vendor/product line there are three major
incridients,
in common to all products is the presence of three protease sub-classes,
we will perform some test
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In addition to all the excellent suggestions to perform seeding, you could also
try sitting drop vapor diffusion, and also different temperatures. Sometimes
moving from 20 to 4 degrees does the trick.
Diana
**
Diana R. Tomchick
Professor
Hi Nikolaus
I completely agree with Claude's comments. Microseeding is the first thing
to try. You would like to find conditions where you *don't *get crystals
without seeding, but you *do *get crystals with seeding. Then, just by
diluting the seed stock, you can control nucleation. Look at
Le 09/09/2019 à 17:22, Nikolas a
écrit :
Dear
crystalgrowers,
I am
currently working with a protein that appeared to be
friendly but turned out it was not the case.
I
Hi Nikolas
The first thing I would try is to solubilise the protein a bit more, maybe by
adding a few % (1-5%) glycerol or ethylene glycol to the growth conditions when
you set up the drops.
The next thing would be to experiment with ratio (protein:precipitant) and drop
size.
Lastly, trial
Dear crystalgrowers,
I am currently working with a protein that appeared to be friendly but
turned out it was not the case.
I found myself to face -in the scale up- the opposite of the usual problem
of nucleation (I really love how this topic finds new ways to make fun of
me). In 24-well plates,
Hi all,
I’m looking for a web applet (preferably javascript) that can be used to show
anisotropic displacement parameters (thermal ellipsoids) for atoms.
Does anyone know of one that supports this? I’ve looked through the usual
suspects but none of them seem to go beyond cartoon/sticks/etc.
Well, trade secret means exactly what it says. We were equally unable to
extort it from the suppliers.
MS fragment search as suggested might work.
Best, BR
From: CCP4 bulletin board On Behalf Of Jorg
Stetefeld
Sent: Monday, September 9, 2019 00:02
To: CCP4BB@JISCMAIL.AC.UK
Subject:
Dear all,
MAX IV in Lund, Sweden has opened the call to receive proposals requesting
beamtime at BioMAX for the period March 2020 - August 2020 for normal proposals
and March 2020- Feb. 2021 for BAG proposals.
In order to submit a new proposal, please visit this page:
Hi Chitra
Needles usually work very well for making seed stocks for random Microseed
Matrix Screening (MMS). Your protein probably crystallizes well, but it is
growing too fast in one direction.
MMS has lots of advantages. If it's going to work it will almost certainly
work within 12 hours.
Hi,
If you reindexed crystal 1, the cell dimensions could be a=61.0, b=133.23,
c=100.34, which would be correspond roughly to doubling the b-cell edge of
crystal 2. Is there a sign of this in the data, i.e. in the current indexing
of crystal form 1 is there a big peak around 0,0,1/2 in the
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