Hi Chitra Needles usually work very well for making seed stocks for random Microseed Matrix Screening (MMS). Your protein probably crystallizes well, but it is growing too fast in one direction.
MMS has lots of advantages. If it's going to work it will almost certainly work within 12 hours. Also, it allows you to control the number of crystals per drop by diluting the seed stock. Another excellent (open-access) paper that gives lots of examples of seeding, cross-seeding, dilution, optimization etc within a defined set of fabs is here: https://scripts.iucr.org/cgi-bin/paper?nj5193 Good luck, Patrick On Sun, Sep 8, 2019 at 1:46 PM David Briggs <[email protected]> wrote: > 4. Matrix microseeding. Make a seed stock from these crystals and then > re-run your primary screens. > > https://www.ncbi.nlm.nih.gov/m/pubmed/25195878/ > > -- > Dr David C. Briggs > Senior Laboratory Research Scientist > Signalling and Structural Biology Lab > The Francis Crick Institute > London, UK > == > about.me/david_briggs > > ------------------------------ > *From:* CCP4 bulletin board <[email protected]> on behalf of chitra > latka <[email protected]> > *Sent:* Sunday, September 8, 2019 12:12:28 PM > *To:* [email protected] <[email protected]> > *Subject:* Re: [ccp4bb] Optimization from needle shaped crystals > > I can share what has worked for my crystals : > > 1. You can put a grid across your condition with same or altered drop > ratios. > > 2. You can try micro seeding. (This has given me the best results so far). > > 3. You can try Hampton's additive screen. > > On Sun, Sep 8, 2019 at 10:09 AM Prem Prakash <[email protected]> wrote: > >> Dear all, >> Sorry for a trivial query. I am trying to Co-crystallize my protein with >> its substrate (peptide) using commercial screenings. In one condition of >> JCSG plus (Molecular Dimension) that contains 0.2 M Magnesium chloride >> hexahydrate, 0.1 M Tris 8.5 50 % v/v Ethylene glycol, I got needle like >> crystals (picture attached). Does anyone have idea to optimize such needles >> into better crystals. I would appreciate all your suggestions. >> >> Thank you >> With kind regards, >> Prem Prakash (Ph.D.) >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> <https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1&data=02%7C01%7C%7C9e5bd9b3c9e7485ed8af08d7344ee01c%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C1%7C637035385686224007&sdata=635CdZ1OnWv%2BoTW6YrzI%2BJaPg64OrVyWzz7p9HiAt2c%3D&reserved=0> >> > > > -- > Regards > Chitra > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1&data=02%7C01%7C%7C9e5bd9b3c9e7485ed8af08d7344ee01c%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C1%7C637035385686234001&sdata=iT7qhIjkuHu1eO%2BqZMthtr4gWL%2B3pI0eYOfJOR0Jm9I%3D&reserved=0> > > The Francis Crick Institute Limited is a registered charity in England and > Wales no. 1140062 and a company registered in England and Wales no. > 06885462, with its registered office at 1 Midland Road London NW1 1AT > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- [email protected] Douglas Instruments Ltd. Douglas House, East Garston, Hungerford, Berkshire, RG17 7HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
