conditions are physiological at least for intracellular bacterial proteins.
Prof. Jon R Sayers FRSB
Dept. of Infection, Immunity & Cardiovascular Disease
University of Sheffield Medical School
Beech Hill Rd
Sheffield S10 2RX
United Kingdom,
Tel +44 (0)114 215 9552
Fax +44 (0) 114 271 3892
Email j.r.s
ing to crystallize the protein with no success so far.
>
> Moreover, my thermofluor assays give very low fluorescence. I use Sypro
> Orange as a fluorophore.
>
>
>
> Suprisingly, a point mutation in the active site (His to Arg) gets rid of
> the issue of contamination and gives me good thermofluor curves. I purify
> the mutant also form IBs.
>
>
>
> Can someone suggest what this "contamination" may be?
>
>
>
> Thank you for your time.
>
>
>
>
>
--
Best wishes
Prof. Jon R Sayers, FRSB
Tel: +44 (0) 114 2159552
Email: j.r.say...@shef.ac.uk
http://www.sheffield.ac.uk/iicd/profiles/sayers
> > ___________
> > phenixbb mailing list
> > pheni...@phenix-online.org
> > http://phenix-online.org/mailman/listinfo/phenixbb
> > Unsubscribe: phenixbb-le...@phenix-online.org
> >
> >
> >
> >
> > UT Southwestern
> >
> >
> > Medical Center
> >
> >
> >
> > The future of medicine, today.
> >
>
>
>
--
Best wishes
Prof. Jon R Sayers, FRSB
Tel: +44 (0) 114 2159552
Email: j.r.say...@shef.ac.uk
http://www.sheffield.ac.uk/iicd/profiles/sayers
n and gives me good thermofluor curves. I purify
> the mutant also form IBs.
>
> Can someone suggest what this "contamination" may be?
>
> Thank you for your time.
>
>
>
--
Best wishes
Prof. Jon R Sayers, FRSB
Tel: +44 (0) 114 2159552
Email: j.r.say...@shef.ac.uk
http://www.sheffield.ac.uk/iicd/profiles/sayers
he Flory Institute for Host-pathogen
Interactions.
You will hold a good honours degree in a relevant subject and a PhD in
macromolecular crystallography. This post is fixed term for 16 months, with
a start date of 1
February
2018 (or as soon as possible thereafter).
--
Professor Jon R Sayers, FRSB
De
--
Best wishes
Prof. Jon R Sayers, FRSB
Tel: +44 (0) 114 2159552
Email: j.r.say...@shef.ac.uk
http://www.sheffield.ac.uk/iicd/profiles/sayers
Are you Mac Users running CCP4 prods under High Sierra?
Sorry if I've missed this in an archive search but as a serial late adopter
of new OS releases, I've resisted upgrading until I know things work.
BW Jon
--
Professor Jon R Sayers, FRSB
Department of Infection, Immunity & Cardiovasc
web site.
https://www.sheffield.ac.uk/jobs. (reference UOS018387). or via:
http://www.jobs.ac.uk/job/BHN194/postdoctoral-research-associate-in-structural-biology/
--
Professor Jon R Sayers, FRSB
Department of Infection, Immunity & Cardiovascular Disease
University of Sheffield Medical School
Beech H
on homologous FENs derived from bacteria and viruses. For more details of
our FEN research see: www.sayers.staff.shef.ac.uk/fen/index.html
--
Professor Jon R Sayers, FRSB
Department of Infection, Immunity & Cardiovascular Disease
University of Sheffield Medical School
Beech Hill Rd
Sheffield
in Trypanosomes. The NMR work will be
carried out in Prof. Jon Waltho's lab and crystallography is supervised by
Dr Pat Baker (both Sheffield).
The posts are both based in Sheffield, UK.
--
Professor Jon R Sayers, FRSB
Department of Infection, Immunity & Cardiovascular Disease
University of Sheffield Med
e both based in Sheffield, UK. Depending on experience, we are
able to appoint at or near the top end of the scale advertised. Feel free
to contact me to discuss.
--
Professor Jon R Sayers, FRSB
Department of Infection, Immunity & Cardiovascular Disease
University of Sheffield Medical School
Beech H
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