Hi, Sorry for asking an off-topic question,
I have recently purified a protein having a molecular weight of 40kDa and
concentration of the protein was 8mg/ml. When I tried to set the protein
for crystallisation using micobatch method, the protein started
precipitating in most of the buffer
Hi Vicky,
I tried to change the ratio of paraffin:silicon oil to 1:2, but still the
protein precipitated. So I tried to do it other way round, I kept
paraffin:silicon oil ratio as 2:1 and so far it has not precipitated. Apart
from that, I separately used the two oils.In silicon oil there was
either using matrix
micro seeding, or change the protein buffer. Perhaps adding a ligand or a
component from your current crystallisation conditions to your protein
stock?
HTH,
Dave
On Fri, 24 Apr 2015 04:02 Prerana G. tracy...@gmail.com wrote:
Dear all,
I am working on a protein (40kDa
Dear all,
We have two data sets of a protein with the following parameters:
1. Space group P212121 a=61.0, b=100.34, c=133.23 No. of molecules in ASU -
2
2. Space group P212121 a=58.33, b=62.86, c=109.45 No. of molecules in ASU -
1
Can we use them as two different structures?
Regards,
Prerana
