[ccp4bb] In Situ Structural Cell Biology | Faculty position at University of Bristol

Dear All, We are very pleased to announce that we are advertising for a permanent Associate Professorship/Senior Lectureship in “in situ structural cell biology”. This focussed advertisement addresses an area that we identified as a strategic priority in our School of Biochemistry at the

Re: [ccp4bb] AW: [ccp4bb] Cold cabinet recommendations for AKTA Pure

rmal working hours. In any case, I do > not expect a response outside those hours. Stay home if you're not involved > in essential work, stay safe.* > > > > > > Newcastle University Biosciences Institute > > Faculty of Medical Sciences > 2nd Floor Cookson Buil

[ccp4bb] Cold cabinet recommendations for AKTA Pure

Dear All Apologies for being wildly off topic, but we're trying to find a decent cold cabinet for 2x AKTA Pure + F9C + sample pump. Does anyone have any recommendations, especially for sliding door models? Cheers, Alan

Re: [ccp4bb] Please recommend linux workstation

Emerald BioStructures http://www.emeraldbiostructures.com/ Tel: 206-780-8914 Cell: 240-479-6575 E-mail: fairman@gmail.com mailto:fairman@gmail.com jfair...@embios.com mailto:jfair...@embios.com -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany

[ccp4bb] first use of synchrotron radiation in PX

Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron? Thanks in advance Alan -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E

[ccp4bb] Superposing nucleic acids

Dear all - is there a convention for superposing nucleic acids duplexes of unrelated sequence? i.e. which atoms should be superposed : C4' of the sugar, P of the backbone, O3'/O5' of the backbone, or some combination thereof? Alan -- Alan Cheung Gene Center Ludwig-Maximilians-University

Re: [ccp4bb] refining against weak data and Table I stats

/ ^\ /` /^. / /\ / / /`/ / . /` / / ' ' ' -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: che...@lmb.uni-muenchen.de

Re: [ccp4bb] refining against weak data and Table I stats

/ ^\ /` /^. / /\ / / /`/ / . /` / / ' ' ' -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: che...@lmb.uni-muenchen.de -- Alan Cheung Gene Center Ludwig-Maximilians-University

[ccp4bb] Collecting native data at an absorption edge

do the lot at the zinc edge wavelength, or should we be worried about radiation damage, and move away from the edge for the native datasets? And would a native dataset for refinement be compromised by friedel pair differences collected at an edge? Alan -- Alan Cheung Gene Center Ludwig

Re: [ccp4bb] Structure example request for large domain movement in crystallo soaking

by this result. My question is, do you have any other example like mine, where domain (or loop) movement is observed _*in crystal*_ during ligand _*soaking*_ procedure? It would be very helpful for me to relate my result to other similar examples. Thank you very much. King regards, Wenhe -- Alan

Re: [ccp4bb] using pymol for making movie of change in protein structure

. Can someone please help in suggesting some other tool and tutorial for the same. Thanks in advance. Regards -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: che...@lmb.uni-muenchen.de

Re: [ccp4bb] 5-Bromo-2′-deoxyuridine monomer in coot and Jligand

Hi Yu - we regularly model BrdU in our DNA chains with the replace residue function in coot. Model a normal thymine in the required position and then centre on the thymine. Then select : Extensions Modelling Replace Fragment and type BRU into the dialog box. (see 5.17.3 of

Re: [ccp4bb] 5-Bromo-2′-deoxyuridine monomer in coot and Jligand

Whoops, i meant Extensions Modelling Replace Residue and not replace fragment. On 24/10/2011 09:43, Alan Cheung wrote: Hi Yu - we regularly model BrdU in our DNA chains with the replace residue function in coot. Model a normal thymine in the required position and then centre

Re: [ccp4bb] how to deal with the DNA in Refmac?

the geometry of DNA to fit my poor density? Or, how to fix the DNA (just like in CNS)? Anyone could share their experience and tricks about dealing with DNA? cheers Xun -- Department of Molecular and Structural Biochemistry North Carolina State University -- Alan Cheung Gene Center Ludwig-Maximilians

Re: [ccp4bb] interface

-- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: [EMAIL PROTECTED]

[ccp4bb] Nucleic acid refinement with Refmac at 3.6A

help? Or should i be using CNS? Alan -- Alan Cheung Gene Center Ludwig-Maximilians-University Feodor-Lynen-Str. 25 81377 Munich Germany Phone: +49-89-2180-76845 Fax: +49-89-2180-76999 E-mail: [EMAIL PROTECTED]