[ccp4bb] problems loading images in iMosflm under CCP4

[Annette Herta Erbse]

Hi Everyone,

I apologize in advance I am probably doing something stupidly wrong, but I have 
problems adding images taken with a Dectris Pilatus  200K detector  into 
iMosflm. The files are .img files. I get the message "Error reading image 
header. Message from Mosflm is Incorrect size of image for detector type MAR". 
But if I look at experiment settings detector it has recognized it as RIPI 
Model 200K and under Experiment it has the beam position, the distance and the 
wavelength right. So it is be able to read the header? I am confused why it 
thinks it is a MAR detector image.

Again I apologize if I am missing something obvious here.
Best - Annette

Voice: ++1-303-492-0528 (office)
Fax: ++1-303-492-8425
Email:  er...@colorado.edu
Office: JSCB C316




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[ccp4bb] Mosaicity histogram in HKL 3000R

[Annette Herta Erbse]

Hi Everyone,


I am seeing something strange with HKL 3000R with regard to the mosaicity 
histogram and was wondering if anyone has come across something like it before. 
From the HKL 2000 manual  I understand that the mosaicity histogram should to 
go through 0 close to 0.5 partiality, to have higher values in the negative 
zone and to go down to close to 0 at +mosaicity/2. I am  working on a Rigaku 
system (XtaLAB MM003 , AFC11 Partial-χ 4-axis goniometer , PILATUS 200K 
detector) and it came with HKL 3000R. I have looked at quite a few different 
crystals from Lysozyme to RNA and in every case the first zone in the histogram 
is the 0 zone and it is (somewhat) close to  0.5 partiality and the histogram 
goes down from there to close to 0 at + mosaicity/2. But the histogram has 
nothing in negative zones. I am trying to understand how that can be and if 
this indicates a problem of some kind. Or maybe I am simply doing something 
stupid wrong? I am using a 3D window that covers about twice the mosaicity. I 
have seen it with data that were collected with 0.25,  0.5 or 1 degree 
oscillation.  I appreciate any advice.

Thanks - Annette

Annette H. Erbse
Department of Chemistry & Biochemistry
University of Colorado at Boulder

Voice: ++1-303-492-0528 (office)
Fax: ++1-303-492-8425
Email:  er...@colorado.edu
Office: JSCB C316

[ccp4bb] ice build up during collection using 4 axis goniometer

[Annette Herta Erbse]

Thank you everyone for all the help.

Gottfried – Thanks for the photo I will give this a try.

Best - Annette

From: p...@uni-greifswald.de [mailto:p...@uni-greifswald.de]
Sent: Friday, September 15, 2017 1:17 AM
To: Annette Herta Erbse
Subject: Re: [ccp4bb] ice build up during collection using 4 axis goniometer

Dear Anette,
  appended is a photo of our AFC12 setup. I had to position the cryo coming 
almost directly from top to be able to use kappa to +- 60° and 2theta to -100°. 
With more hunidity in summer I do get some ice, but it is at the pin below the 
crystal. I have never ovserved a shadow from the ice on the detector and as 
omega proceeds old "beards" of ice melt away.
More pictures only if you like, they are so big.
Good luck
  Gottfried


On Wednesday, 13-09-2017 at 21:54 Annette Herta Erbse wrote:

Dear All,

We have a Rigaku XtalLab with a MM003 generator, a 4 axis goniometer and an 
Oxford 700 cryostream. I have been running into the problem that if I make full 
use of the  4 axis goniometer in the collection strategy  I run into geometries 
where the angles between the pin/Xtal and the Oxford cryo head nozzle are 
smaller than 90 0 and in these positions I start collecting a lot of ice to a 
point were it get's blown off by the cold stream and I have lost  Xtals. I was 
wondering if others have experienced the same and if there is a good way to 
avoid it. At the moment I feel like I simply have to avoid these ranges which 
is a shame since it restricts  the data collection strategy.

I appreciate any advice - Annette

[ccp4bb] ice build up during collection using 4 axis goniometer

[Annette Herta Erbse]

Hi Pius,


I just wanted to say that Rigaku is absolutely helping me trouble shoot this. 
They are really good in providing support. I just posted this here in case 
someone had seen this before and had a solution that I am missing because I 
don't have a huge amount of experience with this yet.

I was thinking along the lines more minds together and more experience work 
better!

Best - Annette

[ccp4bb] ice build up during collection using 4 axis goniometer

[Annette Herta Erbse]

Hi Scott and Henry ,

Thanks you for the fat response. I am using copper pins.
What is interesting for me is that it really only happens at certain 
geometries. As long as I don't move chi I can collect for days, if I set chi to 
let's say 400 and Omega brings the pin to move so that it forms a small angle 
with respect to the nozzle I get ice. In the Oxford cryo steam manual it gives 
too small angles between pin and nozzle as one possible reason for ice buildup. 
I have tried to rearrange the set up as much as possible but have not found a 
good solution for this.

Best - Annette



Voice: ++1-303-492-0528 (office)
Fax: ++1-303-492-8425
Email:  er...@colorado.edu
Office: JSCB C316

[ccp4bb] ice build up during collection using 4 axis goniometer

[Annette Herta Erbse]

Dear All,

We have a Rigaku XtalLab with a MM003 generator, a 4 axis goniometer and an 
Oxford 700 cryostream. I have been running into the problem that if I make full 
use of the  4 axis goniometer in the collection strategy  I run into geometries 
where the angles between the pin/Xtal and the Oxford cryo head nozzle are 
smaller than 90 0 and in these positions I start collecting a lot of ice to a 
point were it get's blown off by the cold stream and I have lost  Xtals. I was 
wondering if others have experienced the same and if there is a good way to 
avoid it. At the moment I feel like I simply have to avoid these ranges which 
is a shame since it restricts  the data collection strategy.

I appreciate any advice - Annette