[ccp4bb] Fwd: [ccp4bb] XSCALE vs scala

Begin forwarded message: From: Graeme Winter Date: March 4, 2011 4/3/11 - 4:51 To: José Trincão Subject: Re: [ccp4bb] XSCALE vs scala Hi Jose, if you are following the usual XDS procedure, the data will have already been scaled by the XDS CORRECT step. XSCALE will perform essentially the

[ccp4bb] XSCALE vs scala

procedure instead of scaling and converting with XSCALE and XDSCONV? Thanks! Jose José Trincão, PhD CQFB@FCT-UNL 2829-516 Caparica, Portugal "It's very hard to make predictions... especially about the future" - Niels Bohr

Re: [ccp4bb] Merging data to increase multiplicity

n > completeness, Rmerge and multiplicity. > Now, is there any reason why I should not just merge all these together and > feed them to scala in order to increase multiplicity? > Am I missing something? > > Thanks for any comments! > > Jose > > > José Trincão, PhD

[ccp4bb] Merging data to increase multiplicity

why I should not just merge all these together and feed them to scala in order to increase multiplicity? Am I missing something? Thanks for any comments! Jose José Trincão, PhD CQFB@FCT-UNL 2829-516 Caparica, Portugal "It's very hard to make predictions... especially about

[ccp4bb] Trouble with P1 data

getting better crystals, is there anything we could try to improve our data, lower our Rfree? Thanks! Jose Trincao José Trincão, PhD c...@fct-unl 2829-516 Caparica, Portugal "It's very hard to make predictions... especially about the future" - Niels Bohr

[ccp4bb] Tcl error - Snow Leopard OSX

Hello all, I upgraded my Macbook Pro to snow leopard and followed William G. Scotts Crystallography on OSX wiki to install CCP4 using Fink. I used the 64bit install with full compilation, not the pre-compiled binaries. When I start ccp4i I get a warning: Application initialization failed: ve

Re: [ccp4bb] Create a dual boot windows-linux machine

Dear Xie, you should seriously consider what Johan Turkenburg suggested: create a 20~30Gb ext3 patition that you mount as / and the rest of the disk as ext3 mounted as /home. That way, if you have to reinstall linux, all your files will be safe. You would end up with the following partitions

Re: [ccp4bb] rescuing crashing-out protein eluted from Nickel column

Hi Jacob, try adding a bit of edta (1mM) to remove any Ni that might come off the column. You could also add some DTT or bME to keep cysteines reduced (careful, add only after the edta!). In my experience the gradient did not work very weel because the protein with have a lot of impurities. Yo