Re: [ccp4bb] Crystallizing a tough target

[Jose A. MARQUEZ]

Dear kavya

There is precedent for this. Check the reference below. This protein was 
crystalized from solution at a concentration of 150 mg/ml. Keep 
concentrating, I guess !


Cell. Volume 98, Issue 4 
<https://www.sciencedirect.com/journal/cell/vol/98/issue/4>, 20 August 
1999, Pages 537-546


https://doi.org/10.1016/S0092-8674(00)81981-9

Best regards

Josan

_
Jose A. Marquez, Senior Scientist
Head of the Crystallization Facility
European Molecular Biology Laboratory, Grenoble.
Delivery address: EMBL, 71, Avenue des Martyrs
38000 Grenoble, France
Postal address: EMBL, 71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/  
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/  
https://htxlab.embl.org/  
_


On 2/5/2024 11:27 AM, kavyashreem wrote:


Dear All,

Has anyone worked on a protein which is highly soluble even at 80mg/ml?

We have one such candidate, which does not precipitate even at 80mg/ml 
instead forms phase separated globules in crystallization plate, which 
eventually hardens over a period of 1 to 1.5 months (which is 
florescent under UV microscope.)


We tried screening at different pH, but failed to get any hits.

Since we got few conditions in which the phase separated globules 
solidified, we focused on them and expanded with 120mg/ml protein, 
still there were not visible precipitates except for the phase 
separation. This has been a challenging target so far. We have tried 
with different constructs, which unfortunately are not soluble!


Does POMs help in such cases? Or do you have any other suggestion.

Thank you

Regards

Kavya





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[ccp4bb] Several Positions available at EMBL Grenoble

[Jose A. MARQUEZ]

Dear All,

We have several job openings at EMBL Grenoble

*/Full-Stack Developer /
*

https://www.embl.org/jobs/position/GR00219

*/Research Technician/*

https://www.embl.org/jobs/position/GR00220

/*Structural Biology Data Platform Developer*/

https://www.embl.org/jobs/position/GR00221
<https://www.embl.org/jobs/position/GR00221>

Best wishes

--
_
Jose A. Marquez, Senior Scientist
Head of the Crystallization Facility
European Molecular Biology Laboratory, Grenoble.
Delivery address: EMBL, 71, Avenue des Martyrs
38000 Grenoble, France
Postal address: EMBL, 71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/  
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/  
https://htxlab.embl.org/  
_




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Re: [ccp4bb] Fragile Crystals

[Jose A. MARQUEZ]

Dear Elizabeth,


/In situ/ data collection is a good approach to try in your case. You 
could use the CrystalDirect technology for automated crystal harvesting 
that is more gentle to crystals than manual harvesting. This is  
available both at EMBL Grenoble and EMBL Hamburg facilities, which offer 
integrated crystallography services in collaboration with the ESRF and 
Petra III synchrotrons.



doi:10.3791/62491.

doi:10.1016/j.crmeth.2021.100102.

doi:10.1107/s0907444912031459.


Best wishes


Josan

_
Jose A. Marquez, Senior Scientist
Head of the Crystallization Facility
European Molecular Biology Laboratory, Grenoble.
Delivery address: EMBL, 71, Avenue des Martyrs
38000 Grenoble, France
Postal address: EMBL, 71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/  
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/  
https://htxlab.embl.org/  
_


On 11/22/2023 5:44 PM, Blake, Morgan Elizabeth wrote:

Hello!

I am a PhD student working on a crystallography project to wrap up my 
dissertation research. I have purified a complex of two proteins, and 
I can consistently grow crystals in 10% PEG3350, 0.2M KSCN, 0.1M 
BIS-TRIS propane pH 7.5. These crystals have sharp edges and can grow 
to a large size (greater than 0.5 mm), but the crystals seem to be 
very fragile. When we open the drops to harvest the crystals, we have 
little time to harvest the crystals before they crack. When we move 
the crystals to a cryoprotectant, over time they start fracturing. 
We've tried using different percentages of glycerol, ethylene glycol, 
PEG400, and oil for cryoprotectants with no success. Needless to say, 
the crystals do not diffract well, with spot patterns that look very 
streaky/mosaic, which I presume is due to the defects that we see in 
harvesting/handling. We have screened for alternate crystallization 
conditions, but we seem to get the same morphology in other 
conditions. Does anyone have suggestions for additives we could use 
post-crystallization to help stabilize our crystals?


Thanks for your advice!



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[ccp4bb] Two positions available at EMBL Grenoble

[Jose A. MARQUEZ]

Dear All,

We have two job openings at EMBL Grenoble

Full-Stack Developer

https://www.embl.org/jobs/position/GR00219

Research Technician

https://www.embl.org/jobs/position/GR00220

Best wishes

_
Jose A. Marquez, Senior Scientist
Head of the Crystallization Facility
European Molecular Biology Laboratory, Grenoble.
Delivery address: EMBL, 71, Avenue des Martyrs
38000 Grenoble, France
Postal address: EMBL, 71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/  
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/  
https://htxlab.embl.org/  
_




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[ccp4bb] Staff Scientist position at EMBL, Grenoble

[Jose A. Marquez]

Dear Colleagues,

Please, feel free to pass along to anyone that might be interested.

*Staff Scientist Position at EMBL, Grenoble*

EMBL is seeking to recruit a Staff Scientist in the Marquez team at the 
EMBL Grenoble unit. The Marquez team operates the High Throughput 
Crystallization Laboratory (HTX Lab) and has a strong focus on 
technology and methods development in macromolecular crystallography 
(CrystalDirect™, CRIMS, Online Crystallography), particularly in the 
area of fragment screening and structure-based drug design. They work 
closely with the EMBL instrumentation and synchrotron diffraction teams 
and apply these technologies to provide services to the wider scientific 
community and for their own research. The HTX lab cooperates in the 
operation of the ESRF MASSIF1 beam line, run by the EMBL - ESRF joint 
structural biology group, where the CrystalDirect technology has been 
recently implemented.


See details and the full job description at: 
https://www.embl.org/jobs/position/GR00203


Applications should be submitted through the www.embl.de/jobs/ portal.

*Deadline for application is April 23rd *.

For questions concerning this position please contact Jose  A. Marquez 
(marq...@embl.fr)


Best regards

Jose A. Marquez

_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/
https://htxlab.embl.fr/
_



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[ccp4bb] New CrystalDirect Plate-to-beam experimental modalities at ESRF MASSIF 1 open for user access through the EMBL HTX lab.

[Jose A. Marquez]

Dear All,

Under the umbrella of the EMBL-ESRF Joint Structural Biology Group a 
collaborative project to upgrade MASSIF-1 and exploit synergies with the 
EMBL High Throughput Crystallization facility (HTX Lab) in Grenoble has 
been established. An integral part was the installation of one of the 
CrystalDirect automatic crystal harvesting robots into the beam line 
environment, enabling new experimental modalities. Thanks to the hard 
work of the EMBL Instrumentation, Synchrotron Crystallography and HTX 
Lab Teams, and the ESRF Structural Biology group, the installation is 
now completed.


We are now opening access to newly developed CrystalDirect plate-to-beam 
experimental modalities at MASSIF-1 via the EMBL HTX Lab. We are 
inviting applications for either collaborative or user access. 
Opportunities for funded access are available through iNEXT Discovery. 
If you are interested in any of the new experiments listed below please 
contact us at h...@embl.fr or mass...@esrf.fr


•    Automated Protein-to-structure pipelines with CrystalDirect 
plate-to-beam cryogenic data collection. Either for full diffraction 
data collection or rapid diffraction-based screening and prioritization 
of initial crystallization hits.


•    Automated high throughput room temperature data collection to help 
identify conformational variability with potential insights into protein 
dynamics.


•    Automated crystal dehydration experiments to modulate crystal 
parameters.


Pure samples can be shipped to the EMBL HTX Lab were crystallization 
experiments will be carried out. Online experimental design and 
real-time access to results is facilitated via the Crystallographic 
Information Management System (CRIMS) web interface. Typical 
experimental workflows include crystallization screening and 
optimization, crystal harvesting and CrystalDirect-plate-to-beam data 
collection at MASSIF-1.


contact   h...@embl.fr or mass...@esrf.fr

Best wishes

_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/
https://htxlab.embl.fr/
_



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[ccp4bb] Software developer position, X-ray based fragment screening

[Jose A. Marquez]

Dear All

A Senior Software Developer position is available at EMBL Grenoble. We 
are looking for a highly motivated person with experience in the 
development of web applications to contribute to  the CRIMS project, 
with particular emphasis on automated crystallography and fragment 
screening pipelines.


See the job description at https://www.embl.org/jobs/position/GR00187

Applications should be submitted through the
www.embl.de/jobs/ portal.

Deadline for applications is Sept  18th.

For questions concerning this position please contact Jose  A. Marquez 
(marq...@embl.fr)


Best regards

Jose A. Marquez

--
_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/
https://htxlab.embl.fr/
_



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[ccp4bb] Research Technician position at EMBL Grenoble

[Jose A. Marquez]

Dear All,

A Research Technician position is available at EMBL Grenoble.

See the job description at: https://www.embl.org/jobs/position/GR00181

Applications should be submitted through the
www.embl.de/jobs/ portal.

Deadline for applications is March 27th.

For questions concerning this position please contact Jose  A.
Marquez (marq...@embl.fr)

Best regards

--
_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/
https://htxlab.embl.fr/
_



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[ccp4bb] Software developer position, EMBL Grenoble

[Jose A. marquez]

Dear All

A software developer position is available in the High Throughput 
Crystallization Laboratory at EMBL Grenoble. We are looking for a highly 
motivated person with experience in frontend development to contribute 
to  the CRIMS Project.


See the job description at https://www.embl.org/jobs/position/GR00177

Applications should be submitted through the
www.embl.de/jobs/ portal.

Deadline for applications is January 30th.

For questions concerning this position please contact Jose  A. Marquez 
(marq...@embl.fr)


Best regards

Jose A. Marquez
_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/ 


https://htxlab.embl.fr/
_



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[ccp4bb] Software developer position, EMBL Grenoble

[Jose A. Marquez]

Dear All

A software developer position is available in the High Throughput 
Crystallization Laboratory at EMBL Grenoble. We are looking for a highly 
motivated person with experience in frontend development to contribute 
to  the CRIMS Project.


See the job description at https://www.embl.org/jobs/position/GR00177

Applications should be submitted through the
www.embl.de/jobs/ portal.

Deadline for applications is January 15th.

For questions concerning this position please contact Jose  A.
Marquez (marq...@embl.fr)

Best regards

--
_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/
https://htxlab.embl.fr/
_



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[ccp4bb] Research Technician position at EMBL Grenoble

[Jose A. Marquez]

Dear All,

A Research Technician position is available in the High Throughput 
Crystallization Laboratory at EMBL Grenoble.


See the job description at: https://www.embl.org/jobs/position/GR00172

Applications should be submitted through the
www.embl.de/jobs/ portal.

Deadline for applications is November 7th.

For questions concerning this position please contact Jose  A.
Marquez (marq...@embl.fr)

Best regards

_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/
https://htxlab.embl.fr/
_



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[ccp4bb] Job posting: Full-Stack DevOps Developer position at EMBL Grenoble

[Jose A. Marquez]

*F**ull-Stack DevOps Developer position at EMBL Grenoble*


EMBL Grenoble is looking for a Full-Stack Software Developer to join the 
Crystallographic Information Management System (CRIMS) project.


https://www.embl.org/jobs/position/GR00169

The Marquez Team operates the High Throughput Crystallization Laboratory 
(HTX Lab), one of the largest facilities of its kind in Europe and has 
developed automated pipelines for macromolecular crystallography and 
fragment screening based on the CrystalDirect technology. The web-based 
Crystallographic Information Management System (CRIMS) supports 
high-throughput operations at EMBL Grenoble, Hamburg and Heidelberg and 
multiple other laboratories in Europe supporting hundreds of scientists. 
CRIMS provides web interfaces for experimental design along with 
automated data capture from laboratory robots and sample tracking over 
the whole crystallography workflow, including communication with 
external data management resources at several Synchrotrons. The Current 
CRIMS team integrates three developers with different profiles.


We are looking for a dynamic and autonomous person, with a capacity to 
take initiatives to integrate and Lead the CRIMS software development 
Team under the supervision of the Head of the HTX Lab. You are a sharp, 
rigorous and methodical person with great problem-solving skills and 
have good interpersonal skills.


If you are interested in this position please apply here 
https://www.embl.org/jobs/position/GR00169


Best regards

Jose A. Marquez
**

--
_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99

https://www.embl.org/groups/marquez/
https://www.embl.org/services-facilities/grenoble/high-throughput-crystallisation/
https://htxlab.embl.fr/
_



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[ccp4bb] PSDI 2021 Virtual Conference ****Registration Open**** Nov 8th-10th, 2021

[Jose A. marquez]

Dear Colleagues,

Following our recent announcement advising you that this year’s PSDI 
2021 Conference would be held virtually (Nov 8^th -10^th , 14:00 pm– 
18:00 pm CET). We are now delighted to invite you to register and sign 
up for the conference.


This year the conference is organized by Merck, ESRF and EMBL. There 
will be a reduced fee (75 Eur) to support the cost of the virtual 
conference platform. We invite you to register via the on-line 
registration form by clicking *_here 
 
(_*If you are registering from within the US or Canada then please click 
*here 
 
*to register). You are welcome to submit abstracts and posters, some of 
which may be selected for oral presentations.


Details on the conference program are available on the *conference 
website* .


We are very much looking forward to welcoming you to PSDI 2021 Virtual 
Meeting if you have any questions or queries then please do not hesitate 
to contact nicola.harda...@hg3.co.uk .


Tel + 44 (0) 1423 529333.

With very best wishes,

On behalf of the organising Committee



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[ccp4bb] ARISE Postdoctoral Opportunities at EMBL

[Jose A. Marquez]

The new EMBL fellowship programme, ARISE (Career Accelerator for 
Research Infrastructure Scientists), is open for applications!
ARISE aims to offer fellowships to experienced professionals with a 
background in science, technology, engineering, or mathematics (STEM) 
who wish to advance technology development in the life sciences and 
receive training to operate life science research infrastructures.
The first call for Fellows opened on 1 Dec and applications can be 
submitted until 31 Jan 2021 (included).
The link to the application portal is available here: 
https://www.embl.org/jobs/position/EF2/ 
<https://www.embl.org/jobs/position/EF2/>


For further information please visit ARISE website 
<https://www.embl.org/training/technology-developers-programme/arise/> and/or 
contact ARISE management team at ar...@embl.org <mailto:ar...@embl.org>


On behalf of the ARISE programme.

--
_____

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
_




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[ccp4bb] Postdoctoral position - Machine learning in fragment screening and structure-based drug design

[Jose A. marquez]

Postdoctoral fellow - Data Scientist

EMBL Grenoble is looking for a Data Scientist to develop innovative 
approaches for the analysis of structure guided drug design data based 
on advanced computing and machine learning technologies. The HTX lab at 
Grenoble provides access to fully automated crystallographic pipelines 
based on the CrystalDirect technology. These pipelines are applied to 
support large scale ligand and fragment screening projects and generate 
tens of terabytes of data yearly. Data and metadata is automatically 
captured by the Crystallographic Information Management System (CRIMS), 
developed by EMBL. We are looking for a highly motivated person with 
experience in the use of computational approaches in structural biology 
and a willingness to contribute towards the development of innovative 
methods for large scale crystallographic data analysis based on advanced 
computing technologies and machine learning with a focus on fragment 
screening and structure guided drug design applications


Information is available at 
https://www.embl.fr/jobs/searchjobs/index.php?ref=GR00155=1


Applications should be submitted through the
www.embl.de/jobs/ portal.

Deadline for applications is November 29th.

For questions concerning this position please contact Jose
Marquez (marq...@embl.fr)

Best regards



Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
_



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[ccp4bb] Research Technician position at EMBL Grenoble

[Jose A. marquez]

A Research Technician position is available in the High Throughput 
Crystallization Laboratory at EMBL Grenoble.


The HTX lab is one of the largest platforms for automated macromolecular 
crystallization screening in Europe and is offering services through 
Instruct and the EC-funded project iNEXT-Discovery. The Marquez Team has 
developed stream-lined pipelines for fragment screening by X-ray 
crystallography based on the CrystalDirect technology for automated 
crystal harvesting and the CRIMS software. These pipelines are applied 
to study targets of biotechnological and biomedical relevance. The 
successful candidate will contribute towards the operation of the HTX 
facility, the development of innovative methods in protein 
crystallography and will participate in research projects.


https://www.embl.fr/jobs/searchjobs/index.php?ref=GR00154=1[]=4


Applications should be submitted through the
www.embl.de/jobs/ portal.

Deadline for applications is November 22nd.

For questions concerning this position please contact Jose
Marquez (marq...@embl.fr)

Best regards



Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
_



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[ccp4bb] Postdoctoral opportunities, Cryo-EM: Structural analysis of alloantibody-HLA complexes.

[Jose A. marquez]

Postdoctoral opportunities: Structural analysis of alloantibody-HLA 
complexes by Cryo-EM.


This is part of a multidisciplinary, collaborative project between the 
Marquez (EMBL Grenoble), Leach (EMBL EBI) and Kosmoliaptsis (Cambridge 
University) laboratories.  The project aim is to understand molecular 
and structural basis of specific alloantibody-HLA interactions and their 
impact on organ transplant rejection. We employ biochemical, 
biophysical, structural and computational techniques to study specific 
alloantibody-HLA interactions that mediate different effector functions. 
This research project is directly motivated from real clinical problems 
and benefits from direct access to a clinical context (Kosmoliaptsis lab)


We are looking for intrinsically motivated, hypothesis-driven 
individuals with a PhD in relevant fields and significant experience in 
structural biology. Experience in single particle analysis by Cryo-EM 
will be he highly appreciated. The candidate will be based in Grenoble 
and travel to the UK for short time periods.


If you are interested please contact us

marq...@embl.fr , a...@ebi.ac.uk 
 , vk...@cam.ac.uk 




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[ccp4bb] Research technician position at EMBL Grenoble

[Jose A. marquez]

A Research Technician position is available in the High Throughput 
Crystallization Laboratory at EMBL Grenoble


The HTX lab is one of the largest platforms for automated macromolecular 
crystallization screening in Europe and is offering services through 
Instruct and the EC-funded project iNEXT-Discovery. The Marquez Team has 
developed stream-lined pipelines for fragment screening by X-ray 
crystallography based on the CrystalDirect technology for automated 
crystal harvesting and the CRIMS software. These pipelines are applied 
to study targets of biotechnological and biomedical relevance. The 
successful candidate will contribute towards the operation of the HTX 
facility, the development of innovative methods in protein 
crystallography and will participate in research projects. Under 
specific circumstances candidates with a PhD and publications who wish 
to change their career path can also be considered. See details attached.


Applications should be submitted through the
www.embl.de/jobs/ portal.

Deadline for applications is August 15th.

For questions concerning this position please contact Jose
Marquez (marq...@embl.fr)

Best regards



Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
_




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EMBL Grenoble - Research Technician - GR00147 - VN.pdf
Description: Adobe PDF document

[ccp4bb] Mail-in protein-to-structure services to support structural biology research related to COVID-19 at the EMBL Grenoble HTX Lab and the joint EMBL-ESRF MX beamlines.

[Jose A. marquez]

*
*

*Mail-in protein-to-structure services to support structural biology 
research related to COVID-19 at the EMBL Grenoble HTX Lab and the joint 
EMBL-ESRF MX beamlines.*


*
*

The ESRF-EMBL Joint Structural Biology Group (JSBG) invite COVID-19 
related proposals that can benefit from the seamless integration of the 
EMBL High throughput Crystallization Facility (HTX Lab) with the highly 
automated JSBG MX beamlines at the ESRF. The pipeline starts with 
mail-in samples and integrates crystallization screening, crystal 
optimization, ligand soaking if required and eventually fragment 
screening with libraries of up to 1200 fragments. Crystals obtained will 
be automatically harvested and passed to the ESRF for automated X-ray 
data collection, with real-time access to experimental results and 
parameters. This pipeline is uniquely suited to support structural 
biology projects in conditions of confinement and restricted access to 
labs. The HTX lab is already accepting crystallization projects on 
COVID-19 related research and three MX beamlines (MASSIF-1 (ID30A-1), 
ID30B and ID23-2) will become operational from mid-May.


Users wishing to benefit from this combined access for COVID-19 
research, should send an e-mail to jsbg-covid...@embl.fr indicating 
interest, whereupon a short application form will be sent for completion.


Jose A. Marquez

Head of the Crystallization Facility

EMBL Grenoble

marq...@embl.fr

Gordon Leonard

Head of the MX group

ESRF

leon...@esrf.fr




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[ccp4bb] Mail-in protein-to-structure services at EMBL Grenoble HTX Lab to support research on COVID-19

[Jose A. Marquez]

*
*

*Mail-in protein-to-structure services at EMBL Grenoble HTX Lab to 
support research on COVID-19.*


The High throughput Crystallization Facility at EMBL Grenoble (HTX Lab) 
provides access to a fully automated protein-to-structure pipeline based 
on the CrystalDirect technology and the CRIMS software. This pipeline 
starts with mail-in samples and integrates crystallization screening, 
crystal optimization and automated harvesting in a continuous and fully 
automated workflow that can be operated by any scientist from a desktop 
computer with connection to the internet. The CRIMS software is able to 
communicate with the ESRF and Petra III synchrotrons to support 
automated and remote X-ray data collection and provides real-time access 
to experimental results and parameters. These capabilities are uniquely 
suited to support structural biology projects in conditions of 
confinement. The HTX lab is accepting projects on COVID-19 related 
research starting April 16th.


The HTX Lab also provides access to dedicated pipelines for the rapid 
analysis of protein-small molecule interactions as well as for large 
scale fragment screening to support development of inhibitors and 
chemical tools. This includes access to two fragment libraries (1200 and 
700 fragments respectively) and automated data processing for hit 
identification.


If you want to benefit from access to the HTX lab for COVID-19 research, 
please send an e-mail to h...@embl.fr indicating the project goals and a 
description of the samples you would be shipping.


Best regards

Jose A. Marquez

_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
_




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[ccp4bb] PostDoc Position at EMBL Grenoble. Structural Biocomputing

[Jose A. Marquez]

PostDoc Position at EMBL Grenoble. Structural Biocomputing

We have an opening for a post-doc in Structural Biocomputing at EMBL
Grenoble. The successful candidate will be working towards implementing
advanced data analysis in the context of fragment screening and X-ray
crystallography pipelines in Grenoble based on the CrystalDirect
technology. This project will be carried out in collaboration with
Kleywegt, Velankar and Leach groups from the European Bioinformatics
Institute (EBI, Hinxton). Applications should be submitted through the
www.embl.de/jobs/ portal.

https://www.embl.de/jobs/searchjobs/index.php?ref=GR_00121=1[]=0[]=0

Deadline for applications is May 6th.

For questions concerning this position please contact José
Marquez(marq...@embl.fr)

Josan



Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
_



GR_00121.pdf
Description: Adobe PDF document
<>

[ccp4bb] Post-doctoral opportunity in structural bioinformatics at EMBL

[Jose A. Marquez]

Post-doctoral opportunity in structural bioinformatics

The Marquez (EMBL-Grenoble), Kleywegt and Velankar (EMBL-EBI) groups
have a potential opening for a post-doctoral fellow in structural
bioinformatics through the EIPOD Fellowship program. If you're
interested, please contact José Marquez (marq...@embl.fr) before
September 7th (including a CV).

The successful candidate will be working in an interdisciplinary project
at both sites involving integration and archiving of high-throughput
crystallography data. Applicants should hold a PhD in physics,
mathematics, (bio-)chemistry, biology or similar, involving the
application of computational methods in the life sciences, and should
have experience with professional scientific software development
(design, implementation, and maintenance).

Requirements:
- fluency in Python and experience developing web-based applications
using the Python Django framework
- experience with object-oriented PHP
- experience with relational databases and SQL
- experience with JavaScript frameworks (such as jQuery)

Experience or familiarity with one or more of the following would also
be advantageous:
- a background in, or a good level of understanding of X-ray crystallography
- one or more higher-level programming languages (e.g., C++, Java, Fortran)
- development of (scientific) data-processing pipelines

For more details on the EIPOD program, see
http://www.embl.de/training/postdocs/08-eipod
-- 
_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
_

<>

[ccp4bb] Post-Doctoral position at EMBL Grenoble

[Jose A. Marquez]

A Post-doctoral position is available at the EMBL Grenoble  Outstation.
Please see

https://www.embl.fr/jobs/searchjobs/index.php?ref=GR_00105=1=%2Fjobs%2Fsearchjobs%2Findex.php%3Fsrch_trm%3D%26list%3DGo

Best regards

Josan

-- 
_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
_

<>

[ccp4bb] Scientist Position: HT crystallography at EMBL Grenoble

[Jose A. Marquez]

Dear colleagues,

We are looking for a experienced crystallographer for an Industrial
Liaison Scientist position at the EMBL Grenoble Outstation. Those
interested please, see the link below.

https://www.embl.fr/jobs/searchjobs/index.php?ref=GR_00107=1=%2Fjobs%2Fsearchjobs%2Findex.php%3Fsrch_trm%3D%26list%3DGo

Applications from scientists in either academia or industry are encouraged.

Best regards

Josan

_

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
_

<>

[ccp4bb] Automated pipelines for compound and fragment screening in Grenoble

[Jose A. Marquez]

Automated pipelines for compound and fragment screening in Grenoble

The EMBL Grenoble, the ESRF and the iNEXT consortium are jointly
offering access to automated pipelines for compound and fragment
screening based on the CrystalDirect* technology and the MASSIF**
beamlines. Access to these pipelines can be obtained through the E.C.
H2020 project iNEXT (www.inext-eu.org). Applications will be reviewed by
the iNEXT scientific review panel in collaboration with the ESRF MX
Beamtime Allocation Panel.

These pipelines are offered in two forms:

Compound Screening Pipeline: Automated screening via X-ray
crystallography of a collection of small molecule ligands identified
through previous assays (i.e in silico, screening, activity assays, SPR,
etc.). Applicants must be able to provide the ligand collection, pure
protein in sufficient amounts and well-established and robust
crystallization conditions.

Fragment Screening pipeline: Automated large scale fragment screening
via X-ray crystallography. The fragment collection will normally be
provided by the Grenoble facility although screening of user provided
libraries is also possible. Applicants must be able to provide pure
protein in sufficient amounts and well-established and robust
crystallization conditions

Additionally, we are also offering access to an Integrated
Macromolecular Crystallography pipeline offering fully automated, remote
controlled access to crystallization screening, optimization of crystal
conditions, automated crystal mounting and cryocooling (based on EMBL'S
CrystalDirect technology and automated (or manual) data collection at
the ESRF MASSIF-1 or MASSIF-3 beamlines.

To apply visit the iNEXT web page (www.inext-eu.org) or contact us.

* Automated harvesting and processing of protein crystals through
laser photoablation  Zander, U., Hoffmann, G., ... & Marquez, J. A.
(2016). Acta Cryst. D72, 454-466.
https://doi.org/10.1107/S2059798316000954

** Svensson O, Monaco S, Popov AN, Nurizzo D, Bowler MW (2015). The
fully automatic characterization and data collection from crystals of
biological macromolecules, Acta Cryst. D71, 1757-1767,
doi:10.1107/S1399004715011918

-- 
_____

Jose A. Marquez Ph.D.
Team Leader, Head of the Crystallization Facility
EMBL Grenoble Outstation
Postal address: European Molecular Biology Laboratory
71, Avenue des Martyrs
CS 90181 38042 Grenoble Cedex 9, France
Delivery address: European Molecular Biology Laboratory
71, Avenue des Martyrs
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
_

<>

[ccp4bb] Funded access to Automated Crystallization Services at EMBL Grenoble

[Jose A. Marquez]

--
--
Funded Access to Automated Crystallization Screening and in plate x-ray 
analysis

at Grenoble
--
--

Access to crystallization services at the High Throughput 
Crystallization Laboratory of the EMBL Grenoble Outstation is supported 
by the E.C. Funded Biostruct-X project. To apply please go to the 
Biostruct-X http://www.biostruct-x.eu/ web page. Selecting Single 
application will open a pane giving access to PP  HTX.

*Application Deadline April 30th.*

Starting in April 2013 and in collaboration with ESRF BM14  beamline the 
services will be extended to include automated evaluation of crystal 
hits through in plate X-ray experiments. For this purpose,* 
*crystallization experiments will be performed in specially developed 
low-background CrystalDirect plates (/Acta Cryst./ 2012. D*68*, 
1393-1399). A crystal pointing tool in CRIMS allows user to select 
through the web the crystals you want to test.



The High Throughput Crystallization ( HTX ) laboratory of the EMBL 
Grenoble outstation is one of the key platforms of the Partnership for 
Structural Biology (PSB) http://www.psb-grenoble.eu/and is located 
next to the European Synchrotron Radiation Facility (ESRF) and the 
Institute Laue Langevin (ILL). This facility offers completely automated 
crystallization screening in hanging and sitting drop set up at two 
temperatures (20°C and 5°C). Results together with all experimental 
parameters are made available to users in real time through the 
CRystallization Information Management System (CRIMS), which includes 
fast and convenient web user interfaces providing advanced tools like 
drop scoring, drop history, navigation through project targets and 
samples and project summaries and a crystal pointing tool to support 
automated in plate x-ray diffraction experiments among others. 
Crystallization plates are made available to users upon requests 
(typically during their next synchrotron visit) and facilities for 
crystal mounting and freezing are provided. Since the start of 
operations the HTX lab in Grenoble has provided crystallization 
screening to more than 650 scientists.



Best regards

Jose A. Marquez

--
_
Jose A. Marquez Ph.D.
Team Leader, Head of Crystallization Facility
EMBL Grenoble Outstation
Postal address:   EMBL Grenoble Outstation, 6 Rue Jules Horowitz,  BP181
38042 Grenoble Cedex 9, France
Delivery address: EMBL Grenoble Outstation, Polygone Scientifique,  6 Rue Jules 
Horowitz
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
__

attachment: marquez.vcf

[ccp4bb] Software developer position

[Jose A. Marquez]

Dear All

A Software Developer position is currently available in my laboratory at 
EMBL Grenoble. You will find the details in the link below or at 
www.embl.org/jobs


http://www.embl.fr/aboutus/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670
http://www.embl.fr/aboutus/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670 
http://www.embl.fr/aboutus/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670


Location:   Grenoble, France
Staff Category: Staff Member
Contract Duration:  1 year
Grading:4, 5 or 6, depending on experience and qualifications
Closing Date:   07 April 2013
Reference number:   GR_00052


Best regards

Josan

--
_
Jose A. Marquez Ph.D.
Team Leader, Head of Crystallization Facility
EMBL Grenoble Outstation
Postal address:   EMBL Grenoble Outstation, 6 Rue Jules Horowitz,  BP181
38042 Grenoble Cedex 9, France
Delivery address: EMBL Grenoble Outstation, Polygone Scientifique,  6 Rue Jules 
Horowitz
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
__

attachment: marquez.vcf

[ccp4bb] Position, Research Technician at EMBL Grenoble

[Jose A. Marquez]

Dear All

A research technician position is currently available in my laboratory 
at EMBL Grenoble. You will find the details in the link below or at 
www.embl.org/jobs


http://www.embl.de/aboutus/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670 
http://www.embl.de/aboutus/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670


Location:   Grenoble, France
Staff Category: Staff Member
Contract Duration:  3 years
Grading:4, 5 or 6, depending on experience and qualifications
Closing Date:   25 November 2012
Reference number:   GR_00047



Best regards
Josan

--
_
Jose A. Marquez Ph.D.
Team Leader, Head of Crystallization Facility
EMBL Grenoble Outstation
Postal address:   EMBL Grenoble Outstation, 6 Rue Jules Horowitz,  BP181
38042 Grenoble Cedex 9, France
Delivery address: EMBL Grenoble Outstation, Polygone Scientifique,  6 Rue Jules 
Horowitz
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://embl.fr/htxlab/
__

attachment: marquez.vcf

[ccp4bb] Position available: Research Technician, High Throughput Crystallisation

[Jose A. Marquez]

Dear All,

A Research Technician position is available at the High Throughput 
Crystallisation laboratory of the EMBL Grenoble Outstation.

You will find the details in the link below.

http://www.embl.de/aboutus/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670 

http://www.embl.de/aboutus/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670 



Best regards
Josan

--
_
Jose A. Marquez Ph.D.
The High Throughput Crystallization Laboratory
EMBL Grenoble Outstation
Postal address:   EMBL Grenoble Outstation, 6 Rue Jules Horowitz,  BP181
38042 Grenoble Cedex 9, France
Delivery address: EMBL Grenoble Outstation, Polygone Scientifique,  6 
Rue Jules Horowitz

38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://htxlab.embl.fr
__

[ccp4bb] Position available: Research Technician for High Throughput Crystallisation

[Jose A. Marquez]

 A Research Technician position is available at the High Throughput 
Crystallisation laboratory of the EMBL Grenoble Outstation.

You will find the details in the link below.

https://intranet.embl.de/hr/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670 
https://intranet.embl.de/hr/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670


Best regards
Josan

--
_
Jose A. Marquez Ph.D.
The High Throughput Crystallization Laboratory
EMBL Grenoble Outstation
Postal address:   EMBL Grenoble Outstation, 6 Rue Jules Horowitz,  BP181
38042 Grenoble Cedex 9, France
Delivery address: EMBL Grenoble Outstation, Polygone Scientifique,  6 Rue Jules 
Horowitz
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://htxlab.embl.fr
__

attachment: marquez.vcf

[ccp4bb] Position available: Research Technician for High Throughput Crystallisation

[Jose A. Marquez]

 Dear All,

A Research Technician position is available at the High Throughput 
Crystallisation laboratory of the EMBL Grenoble Outstation.

You will find the details in the link below.

http://www.embl.de/aboutus/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670 
http://www.embl.de/aboutus/jobs/searchjobs/index.php?newlang=1newms=srsearchregion=670


Best regards
Josan

--
_
Jose A. Marquez Ph.D.
The High Throughput Crystallization Laboratory
EMBL Grenoble Outstation
Postal address:   EMBL Grenoble Outstation, 6 Rue Jules Horowitz,  BP181
38042 Grenoble Cedex 9, France
Delivery address: EMBL Grenoble Outstation, Polygone Scientifique,  6 Rue Jules 
Horowitz
38000 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://htxlab.embl.fr
__

attachment: marquez.vcf

[ccp4bb] Postdoctoral Position at EMBL, Grenoble

[Jose A. Marquez]

 Dear All,

A postdoctoral position is available at the EMBL Grenoble Outstation.
You will find the details in the link below

http://ig14.i-grasp.com//fe/tpl_embl01.asp?newms=jjid=41107aid=15470

Best regards
Josan

_
Jose A. Marquez Ph.D.
The High Throughput Crystallization Laboratory
EMBL Grenoble Outstation
Postal address:   EMBL Grenoble Outstation, 6 Rue Jules Horowitz,  BP181
38042 Grenoble Cedex 9, France
Delivery address: EMBL Grenoble Outstation, Polygone Scientifique,  6 Rue Jules 
Horowitz
38042 Grenoble, France
Phone +33 (0)476 20 74 25
Fax. +33 (0)476 20 71 99
https://htxlab.embl.fr
__

attachment: marquez.vcf

[ccp4bb] Postdoctoral position at EMBL Grenoble

[Jose A. Marquez]

*Postdoctoral fellowship at the High Throughput Crystallization 
laboratory of the EMBL Grenoble Outstation*


*EMBL site:* EMBL Grenoble

*Commencing date:* As soon as possible

*Job description:* The High Throughput Crystallization laboratory at the 
EMBL Grenoble Outstation is offering a posdoctoral position. The HTX Lab 
is actively involved in the development of new methods and concepts in 
macromolecular crystallography and works in close co-ordination with the 
high-throughput protein expression and synchrotron instrumentation 
groups at the outstation. The selected candidate will participate in the 
Eurostars funded Crystal Clear project, whose aim is to further develop 
a novel and promising approach to protein crystallization in 
collaboration with an industrial partner. He/she will work on 
crystallisation using the new method and determining crystal diffraction 
quality and will participate in the research projects of the laboratory. 
He/She will also coordinate activities with other Eurostar participants


*Qualifications and experience:* The ideal candidate will have a PhD in 
structural biology or a related subject. The candidate should have 
significant experience in protein crystallization as well as good 
knowledge of X-ray crystallography methods. He/she should have good 
communication skills; strong motivation and excellent scientific skills 
are essential, as well as willingness to work effectively alone and in a 
team. Desire to work in an multicultural  interdisciplinary environment 
is essential.


*Contract:* An initial contract of 1 year will be offered to the 
successful candidate. This can be renewed, depending on circumstances at 
the time of review.


*Web page:* http://www.embl-grenoble.fr/

EMBL is an inclusive, equal opportunity employer offering attractive 
conditions and benefits appropriate to an international research 
organisation.


Please note that EMBL does not return CVs or attached documents to 
applicants.


*To apply, please email a cover letter, CV (in English) and contact 
information of three professional references quoting ref. no. W/09/PD/01 
in the subject line, to: applicat...@embl.de mailto:applicat...@embl.de *


Personnel, EMBL, Postfach 10.2209, 69012 Heidelberg, Germany.

*Fax: +49 6221 387555*   *E-mail:*  applicat...@embl.de 
mailto:applicat...@embl.de


--
_
Jose A. Marquez Ph.D.
The High Throughput Crystallization Laboratory 
EMBL Grenoble Outstation 
Postal address:   EMBL Grenoble Outstation, 6 Rue Jules Horowitz,  BP181
38042 Grenoble Cedex 9, France 
Delivery address: EMBL Grenoble Outstation, Polygone Scientifique,  6 Rue Jules Horowitz
38042 Grenoble, France 
Phone +33 (0)476 20 74 25 
Fax. +33 (0)476 20 71 99 
https://htxlab.embl.fr

__

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