[ccp4bb] Joint Instruct-iNEXT Course - September 25-30 - Oeiras (Portugal)

Cordeiro (FC-UL) and Ricardo Louro (ITQB NOVA) -- * Jose Artur Brito, PhD * * * * Membrane Protein Crystallography

[ccp4bb] Joint Instruct-iNEXT Course - September 25-30 - Oeiras (Portugal)

rdeiro (FC-UL) Ricardo Louro (ITQB NOVA) -- **** * Jose Artur Brito, PhD * * * * Membrane Protein Crystallography

Re: [ccp4bb] Cysteine-S-Iodine interaction

HTH, Jose --- **** * Jose Artur Brito, PhD * * * * Membrane Protein Crystallography Laboratory * * Ins

Re: [ccp4bb] Fusion proteins to solubilize your protein

and the density of the protein of interest...) Is there a way to get this data from the PDB? Here is my list so far: - MBP - GFP - TRX - Lysozyme - GST Thanks -- ** * Jose Artur Brito, PhD * * Research Fellow

Re: [ccp4bb] Cysteine sulfenic acid in coot

Dear Sudipta, for me, what works best is the following: - centre on the cysteine you want to "mutate"; - Extensions -> Modelling -> Replace residue; - enter the appropriate code (for cysteine sulfenic acid is "CSX"). This way, the chain ID, numbering, etc, is all kept. HTH, best, Jose

Re: [ccp4bb] CYS modification and choice of PEG

Dear Antonio, we have seen this type of modifications in some of our structures. The modification of the cysteine (to cysteine-sulfenic, sulfinic or sulfonic acid) usually arises from exposure to oxygen during crystallization. We managed to prevent this by either adding TCEP to the protein

[ccp4bb] Unusual electron density - any guesses??

Dear All, I'm refining an X-ray structure to 1.6A resolution in BUSTER-TNT v2.10. The model is pretty much finished but I see a strange electron density that I can't imagine what it is. Please take a look at four snapshots in http://www.itqb.unl.pt/~jbrito/ITQB/ . Any pointers/guesses are

[ccp4bb] Course “Structure and Function of Membrane Proteins - a multidisciplinary approach”

Dear colleagues, A FINAL announcement about the 4-day course “Structure and Function of Membrane Proteins- a multidisciplinary approach”. The course is funded by Instruct and BioStruct-X and will be held at ITQB, Oeiras (nearby Lisbon), Portugal, from 5 to 8 November 2013. The course will

[ccp4bb] Off Topic: Isothermal Titration Calorimetry (ITC)

has experience with these two equipments? Would you prefer one over the other (please state your reasons)? Would you suggest another equipment/brand for the ITC (like the NanoITC from TA Instruments)? Thanks in advance, Jose Artur Brito -- * José

Re: [ccp4bb] Multiple active or allosteric pocket allignment

Hi Jay. It's ProFIT and you can find it at http://www.bioinf.org.uk/software/profit/ Hope this helps, Jose Jayashankar wrote: (snip) -- * Please note change in telephone number ? José Artur Brito, B.Sc. (Eng.), PhD Student Membrane Protein