Dear All,
sorry for this off-topic questions but I would like to have some
feed-back from you on Isothermal Titration Calorimetry (ITC) equipments.
We have a very nice quotation for an iTC200 from GE Healthcare. We
wanted this one because it uses ~200uL sample per measurement (really
nice when your dealing with "precious" samples, ie., proteins with low
expression yields). However, I was told that, although consuming much
less sample, is not as good (sensitivity, mixing issues, bubbles, ...),
as the VP-ITC (it uses ~1.4mL per measurement, seven times more than the
iTC200).
Does anyone has experience with these two equipments? Would you prefer
one over the other (please state your reasons)? Would you suggest
another equipment/brand for the ITC (like the NanoITC from TA Instruments)?
Thanks in advance,
Jose Artur Brito
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* José Artur Brito, PhD *
* *
* Post-Doctoral Fellow *
* Membrane Protein Crystallography Lab *
* Instituto de Tecnologia Química e Biológica *
* Oeiras - Portugal *
* *
* Tel.: +351.21.446.97.61 *
* Fax: +351.21.443.36.44 *
* *
* E-mail: [email protected] *
* URL: http://mx.itqb.unl.pt *
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