[ccp4bb]

A back of the envellope calculation shows that your asymmetric unit contains most likely 4 molecules with Vm = 2.16 A3/Da, corresponding to 43% solvent. Searching for 16 molecules is thus nonsense. Remy Loris Vrije Universiteit Brussel On 17/08/14 08:54, Avisek Mondal wrote: Hello everyone, i

Re: [ccp4bb] Structure Refinement

. Also: there cannot be three molecules in the unit cell as this space group has six asymmetric units. You probably mean three molecules in the asymmetric unit. Since crystallography is an exact science, one needs to be correct. Remy Loris Vrije Universiteit Brussel On 15/02/13 21:35

Re: [ccp4bb] side chain density

remove the side chain atoms (but keep the residue name). And that is the same as what you do with a whole loop that is disordered. I think it is lso the most common practice in deposited structures. Remy Loris Vrije Universiteit Brussel On 09/11/12 20:22, Faisal Tarique wrote: Dear all i have

Re: [ccp4bb] MR solution

worry me is the apparent special position that you obtain for the translation solution. Are you sure you do not have a higher symmetry or cetered space group? Remy Loris Vrije Universiteit Brussel and VIB On 07/11/12 17:39, rui wrote: Hi, Dear group, I recently collected a dataset about 2.5

Re: [ccp4bb] Problem with making PDB from Coot

Seconday structure assignement by Pymol is poor and unreliable to say the least. This behavior is very common. Best is to assign secondary structure using other software and then tell Pymol explicitely from where to where the starnds and helices run Remy Loris Vriej universiteit Brussel

Re: [ccp4bb] do you think it is interesting?

This may be somethng similar Domain swapping of a llama VHH domain builds a crystal-wide beta-sheet structure. Spinelli S, Desmyter A, Frenken L, Verrips T, Tegoni M, Cambillau C. FEBS Lett. 2004 Apr 23;564(1-2):35-40. Remy Loris Vrije Universiteit Brussel On 18/06/12 15:49, anna anna wrote

Re: [ccp4bb] Off-topic: Supplying PDB file to reviewers

years that I am active in science I had only a single case where I genuinely believe a referee misused his position. This to say that 99.99% of the referee reports are honest, although not necessarily in agreement with your own vision. Remy Loris Vrije Universiteit Brussel and VIB On 19/04

Re: [ccp4bb] MR problem

but rejected by the program because (for example) it has just one clash too many. Remy Loris Vrije Universiteit Brussel and VIB On 04/12/11 05:18, Ping Wang wrote: Dear all, Recently I have a dataset with a protein complex including two proteins. Each structure of the single protein

Re: [ccp4bb] MR problem

by the program because (for example) it has just one clash too many. Remy Loris Vrije Universiteit Brussel and VIB On 04/12/11 05:18, Ping Wang wrote: Dear all, Recently I have a dataset with a protein complex including two proteins. Each structure of the single protein is available. When I

Re: [ccp4bb] how to Collecting Data from Long Unit Cell Axes ?

Ideally you should rotate your crystal around the long axis during data collection. This is however easier said than done since often such crystals grow a plates with the long axis perpendicular to the plane of the plate. As long as the long axis is more or less oriented perpendicular to the

[ccp4bb] Off-topic: chemical modification on thiol groups

for an experiment asked by a referee necessary to confirms or reject a hypothesis that results from a crystal structure. For this experiment it is essential that the reaction is not reversible (so no S-S bond formation). Remy Loris Vrije Universiteit Brussel

Re: [ccp4bb] peptide for co-crystallization

we even crystallized a complex with peptide that was only supposed to be 80% pure (and very nice electron density, crystals diffracting to 1.7 A). Remy Loris Vrije Universiteit Brussel Daniel Jin schreef: Hi, We would like to order two peptides for co-crystallizaiton. I notice

[ccp4bb] Phd studentship in structural biology and biophysics

A Phd studentship is available in the group of Prof. Remy Loris to work on the structural biology and biophysics of the MazEF toxin-antitoxin module from Staphylococcus aureus. The project centres around crystal structure determination of the MazEF module and its complexes, interaction studies

Re: [ccp4bb] is it Ok to freeze

improve it Remy Loris Vrije Univesiteit Brussel Jayashankar wrote: Dear Scientists and Friends, I am not sure, whether organic crystals need to be in cryo stream necessarily during data collection from an in house xray machine . How most of the organic crystals have been solved mostly

[ccp4bb] Opening for a X-ray facility/computing manager

the crystallographic and NMR computing facilities and reassuring of data back-up - Maintenance of the in-house X-ray data collection facilities - Organizing and streamlining synchrotron trips Interested candidates should send their cv to Remy Loris ([EMAIL PROTECTED]) together with the names

Re: [ccp4bb] glycosylation sites]

I think you need to look at this paper, although already quite old: Imberty A, Pérez S. Stereochemistry of the N-glycosylation sites in glycoproteins. Protein Eng. 1995 Jul;8(7):699-709. Remy Loris Vrije Universiteit Brussel Ronnie WEi wrote: I was asked this question by a colleague. Has