-- Forwarded message --
From: wu donghui wdh0...@gmail.com
Date: Mon, Mar 3, 2014 at 7:07 PM
Subject: Re: [ccp4bb] CCP4-6.4.0 source code building failed in Mac OS X
10.8.5
To: Marcin Wojdyr marcin.woj...@diamond.ac.uk
Dear Marcin,
Yes, I set CC=gcc-4.2.1 in cj.rc file or type
-- Forwarded message --
From: wu donghui wdh0...@gmail.com
Date: Mon, Mar 3, 2014 at 10:44 PM
Subject: Re: [ccp4bb] CCP4-6.4.0 source code building failed in Mac OS X
10.8.5
To: Marcin Wojdyr woj...@gmail.com
Dear Marcin,
The reason that I want to build from source
take a look into config.log to read the error message
why your gcc-compiler does not work? You should start at the end of the
log file and scroll backwards until you find the error message.
Best,
Tim
On 03/03/2014 03:51 PM, wu donghui wrote:
-- Forwarded message --
From
Dear all,
I want to know your suggestions about current protein sequence alignment
programs. It seems that different programs give different alignment results
such as from analysis of Clustal W and MULTALIN. Thanks for any input or
comments.
Best regards,
Donghui
Dear all,
I need a script to renumber my image. My initial image number ranges from
*_1081.img to *_1440.img. There are 360 images in total. I want to renumber
these images with the ranges from *_361.img to *_720.img, that means every
initial image-720, but I don't know how to do it. Below is my
:03 AM, wu donghui wdh0...@gmail.com wrote:
Dear all,
Recently I collected several data sets at 13B1 Taiwan beamline with Q315r
detector. It's no problem to index these datasets using mosflm, but Rms
residual and weighted residual is high. Here I want to try XDS to play my
data. I downloaded
. In your case, the file
header tells you that you have binned data, with 3072 x 3072 pixels and a
pixel size of 0.10259, and this is the information you need to give XDS.
Yours,
Marian Szebenyi
MacCHESS
wu donghui wrote:
Dear all,
Thank you very much for your valuable inputs
this helps,
Tim
On Wed, Dec 08, 2010 at 10:53:33PM +0800, wu donghui wrote:
Dear all,
Thank you very much for your valuable inputs. This is an update based from
the suggestions.
Following Pierre's suggestion, I installed xdsme (
http://code.google.com/p/xdsme/) and run it. xdsme extracts
of wu donghui
Sent: Wed 12/8/2010 4:40 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] How to use XDS programme to process data collected at
Q315r detector
Marian,
It seems that XDS does matter detector type Q315 or Q315r. Same crystals
when collected at ESRF Beamline 23-1 using Q315
Dear all,
Recently I collected several data sets at 13B1 Taiwan beamline with Q315r
detector. It's no problem to index these datasets using mosflm, but Rms
residual and weighted residual is high. Here I want to try XDS to play my
data. I downloaded a template example as below.
It does not look like protein crystal and IZIT staining is not reliable in
determining protein or other. Mostly it is like detergent or PEG crystal or
quasi-crystal.
Good luck
Wu
2010/8/31 qiangm zhang zhangqia...@gmail.com
Hi all,
I got a crystal of one membrane protein (~60kD) from
Hi ccp4ers,
I found a problem when I used SHARP 2.6.0 Sushi 3.8.0 to search new heavy
atom sites from 2 identified sites from SnB. Sharp can output new identified
site lists however with all new identified sites set distance at zero with
the 2 sites and strangely the 2 sites all output as G0
Hi ccp4bbers,
I want to know if anyone has any experience about seeding (streak seeding or
microseeding) in microbatch under oil crystallization. I wonder if the oil
might block or wipe away the seeds if cat whisker is used for streak
seeding. Thank you for your input ahead.
Best regards,
2177994, VAT Reg. GB 480 7371 36
*From:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *On Behalf Of *wu
donghui
*Sent:* 19 May 2010 10:29
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] About seeding in microbatch under oil crystallization
Hi ccp4bbers,
I want to know
Two cents are added here.
First, try P2 as somethimes systematic absence along b axis is misleading
due to weak diffraction or pseduo translation.
Second, try P1.
Good luck,
Donghui
On Tue, Jan 26, 2010 at 1:50 AM, Michele Lunelli efu...@yahoo.it wrote:
Dear all,
I am trying to solve a
Dear CCP4ers,
Recently we have solved two structures from E.coli in high resolution, which
have bound two different ligands tightly already. Here I want to know if
there is any program which can let us model the structure of our apo
proteins confidently. Thanks ahead for any comment and input.
region moves upon ligand binding. Any recommendation for any program
which can do this task well. Thanks ahead.
Donghui
On Sun, Aug 9, 2009 at 8:37 PM, wu donghui wdh0...@gmail.com wrote:
Dear CCP4ers,
Recently we have solved two structures from E.coli in high resolution,
which have bound two
Diana,
We already got a new Art Robbins Phoenix crystallization robot recently and
very friendly as time consuming per plate is concerned. Theoretically your
strategy of aspirating one sample enough and dispensing into several 96 well
plate is feasible. However it should depend on the protocol
You might have to test if it is salt/detergent or protein crystal first. If
it is really protein crystal, never give up and for needle crystal,
generally it is difficult to optimze, however in most cases, the diffraction
is well enough. If it is protein crystal, as some researcher has mentioned,
Hi Chen,
In this case, it seems that linker region is of great importance for the
proper folding of the two linked domains. I have not much experience in
linker region design, generally use (GS)5-10 times. However it depends on
individual case. Anyone who has successful experience in linker
Dear all,
Recently, I got a crystallization condition as 0.1 M Bis tris ph 6.5,
1.3Mlithium sulfate,
0.1M NaCl, the shape of crystals is needle cluster, very difficult to grow
bigger, microseeding does not work, then I tried macroseeding, and found
crystal can grow bigger and rod like. However as
On Tue, Feb 26, 2008 at 9:30 PM, wu donghui [EMAIL PROTECTED] wrote:
Dear all,
Recently, I got a crystallization condition as 0.1 M Bis tris ph 6.5,
1.3M lithium sulfate, 0.1M NaCl, the shape of crystals is needle
cluster, very difficult to grow bigger, microseeding does not work, then I
Dear all,
I just get into touch with x-ray crystallography, and I made a big
discovery that there is no C21 space group in monoclinic system, which
system only contains P2, P21 and C2 groups. Even in the entire 65 chiral
space group, there is no space for this group. I made an assumption that if
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