Hi Deepanshu,
how complete the data set? What's completeness across resolution zones?
Systematically missing reflections can have systematic impact in real space
(maps). I've seen entire ligands or domains disappear due to missing
low-resolution data.
Just another check-point to consider..
Good
Dear Deepanshu,
Is the primary sequence in the neighbourhood of your missing strands conserved?
Perhaps that part differs in your structure relative to the search model. Have
you tried omitting the region from MR and rerunning refinement without to see
if some new density pops up?
If not,
Dear all,
I am working on a protein complex. After many efforts, I obtained crystals
from one of the refinement screen (which took few weeks to grow) and I got
a 3 Å dataset at synchrotron. I scaled the dataset in P21 spacegroup (which
is also confirmed by Zanuda and Pointless). There is no
Hello Rajesh
We serendipitously encountered three cases where a contaminating fungus
led to proteolysis of the proteins, which was required for their
crystallization. We identified the fungus by sequencing a little bit of its
genome, and found that it is Penicillium!
Here are two of the
Subject: [ccp4bb] Structure with missing density
Dear BB,
I apologize for the off topic. But I strongly believe this is the right place
to ask my question.
We are working on a protein that is 300 amino acids in length. After many
efforts, we could solve the structure at 1.60 Å resolution
Hi,
Long crystallization times and irreproducibility together with missing
residues points strongly to some slow proteolysis in your crystal drops
that eventually generated a crystallizable fragment. A lot of people
actually do this on purpose by adding proteases to generate stable
fragments.
Dear BB,
I apologize for the off topic. But I strongly believe this is the right place
to ask my question.
We are working on a protein that is 300 amino acids in length. After many
efforts, we could solve the structure at 1.60 Å resolution. It took almost 10
months to get this crystal and we
