[ccp4bb] About seeding in microbatch under oil crystallization

2010-05-19 Thread wu donghui
Hi ccp4bbers, I want to know if anyone has any experience about seeding (streak seeding or microseeding) in microbatch under oil crystallization. I wonder if the oil might block or wipe away the seeds if cat whisker is used for streak seeding. Thank you for your input ahead. Best regards,

Re: [ccp4bb] About seeding in microbatch under oil crystallization

2010-05-19 Thread Harm Otten
Hello Donghui we used a robot to setup the batch crystallization screens with some microseed stock solution and the relevant protein and reservoir stocks. That worked fine. I only mention a collegues work here, so if there are more detailed questions, I will approach him. Good luck, Harm On Wed,

Re: [ccp4bb] About seeding in microbatch under oil crystallization

2010-05-19 Thread wu donghui
2177994, VAT Reg. GB 480 7371 36 *From:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *On Behalf Of *wu donghui *Sent:* 19 May 2010 10:29 *To:* CCP4BB@JISCMAIL.AC.UK *Subject:* [ccp4bb] About seeding in microbatch under oil crystallization Hi ccp4bbers, I want to know

Re: [ccp4bb] About seeding in microbatch under oil crystallization

2010-05-19 Thread Ravindra Makde
/10, wu donghui wdh0...@gmail.com wrote: From: wu donghui wdh0...@gmail.com Subject: [ccp4bb] About seeding in microbatch under oil crystallization To: CCP4BB@JISCMAIL.AC.UK Date: Wednesday, May 19, 2010, 5:28 AM Hi ccp4bbers,   I want to know if anyone has any experience about seeding (streak