To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Blob Game
Dear Crystallographers,
I've got a strange blob hanging off an arginine--see attached. Any ideas?
Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES, CaCl2,
NaCl, Li2SO4. There are really no other side chains which could be close
Ethanol? You weren't setting up drops with liquor on your breath, were you?
I can't remember.
JPK
On 02/23/2015 12:20 PM, Keller, Jacob wrote:
Dear Crystallographers,
I've got a strange blob hanging off an arginine--see attached. Any ideas?
Nothing weird in the prep; cryst+prot conditions:
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Keller,
Jacob
Sent: Monday, February 23, 2015 12:21 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Blob Game
Dear Crystallographers,
I've got a strange blob hanging off an arginine--see attached. Any ideas
,
Jacob
Sent: Monday, February 23, 2015 12:21 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Blob Game
Dear Crystallographers,
I've got a strange blob hanging off an arginine--see attached. Any ideas?
Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES, CaCl2,
NaCl, Li2SO4
Ethanol? You weren't setting up drops with liquor on your breath, were you?
On 02/23/2015 12:20 PM, Keller, Jacob wrote:
Dear Crystallographers,
I've got a strange blob hanging off an arginine--see attached. Any ideas?
Nothing weird in the prep; cryst+prot conditions: NH4SO4, TRIS/HEPES,
@JISCMAIL.AC.UK] on behalf of Keller, Jacob
[kell...@janelia.hhmi.org]
Sent: Monday, February 23, 2015 8:53 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Blob Game
Ethanol? You weren't setting up drops with liquor on your breath, were you
I can't remember.
Ahah, so you did have some liquor
Just a little mother liquor. I am seeing what SO4 does in refinement right now.
JPK
-Original Message-
From: Boaz Shaanan [mailto:bshaa...@bgu.ac.il]
Sent: Monday, February 23, 2015 2:50 PM
To: Keller, Jacob; CCP4BB@JISCMAIL.AC.UK
Subject: RE: [ccp4bb] Blob Game
Boaz Shaanan, Ph.D
on 23/2/15 6:57 PM, jens Preben Morth j.p.mo...@ncmm.uio.no wrote:
a horrible water molecule would fit in there :-)
On 23/02/15 19:42, Keller, Jacob wrote:
A little more info:
-Resolution is 1.75 Ang at edge of detector, but I/sig was still ~3;
pushing integration to corners gives 1.66
