Re: [ccp4bb] AW: [ccp4bb] Cold cabinet recommendations for AKTA Pure
Thanks everyone for recommendations. The AKTA currently lives in a cold room hence all of our purifications have been optimised at 4C, and i'm reluctant to transition them to RT. Also, most of our columns are not jacketed. On Sun, 21 Jun 2020 at 18:19, Boniecki, Michal wrote: > Hi Alan, > I think new AKTA collector can be refrigerated. If this is not an option, > consider putting only collector in the cold cabinet and cooling down the > buffers. Also, you can put only columns and fraction collector inside > cabinet which has side port for such use. Although you will have to use > small diameter out tubing to avoid sample dilution when use like this. > System live will be shorter inside cold cabinet due to increased humidity. > > Michal > > On Jun 21, 2020, at 11:10, Hughes, Jonathan < > jon.hug...@bot3.bio.uni-giessen.de> wrote: > > > CAUTION: This email originated from outside of the University of > Saskatchewan. Do not click links or open attachments unless you recognize > the sender and know the content is safe. If in doubt, please forward > suspicious emails to phish...@usask.ca > > hi alan, > > why do you want to put the thing in a cold cabinet? if you have protease > problems, there are better ways to get rid of them than exploiting Q10. on > the other hand, you'll increase viscosity and thus slow down the flow > rates. also, the high humidity will reduce the lifetime of your Äkta > dramatically (ours has been running almost continuously for 20 years at > room temperature and is still going strong [thanks to tina!]). if you > really to need to keep the sample at 4 °C, you can put the buffer(s) on ice > and cool the column with a jacket. > > best > > jon > > > > *Von:* CCP4 bulletin board *Im Auftrag von *Paula > Salgado > *Gesendet:* Sonntag, 21. Juni 2020 18:50 > *An:* CCP4BB@JISCMAIL.AC.UK > *Betreff:* Re: [ccp4bb] Cold cabinet recommendations for AKTA Pure > > > > Hi Alan > > > > We got one a few years back, from Fisher, although I think Thermo are the > manufacturer's: > > > > Refrigerator FRCR4504W Forma chromatography THERMO SCIENTIFIC FORMA > FRCR4504W > > > > They also arranged for a heavy metal shelf to hold the AKTA as the normal > shelves won't hold the weight. > > > > We only have one AKTA Pure there, with a fraction collector and the rest > of the cabinet holds all the columns. > > > > Hope that helps > > Paula > > > > > > === > > Dr Paula S. Salgado > Senior Lecturer in Macromolecular Crystallography > > Molecular Mechanisms of Life Lead > > *During the current COVID-19 crisis, my working hours tend to focus on > early afternoons and evenings, so please bear with me if my reply is > delayed and if I email outside your normal working hours. In any case, I do > not expect a response outside those hours. Stay home if you're not involved > in essential work, stay safe.* > > > > > > Newcastle University Biosciences Institute > > Faculty of Medical Sciences > 2nd Floor Cookson Building > Newcastle University > Newcastle upon Tyne, NE2 4HH, UK > > Tel: +44 (0)191 208 7432 > Email: paula.salg...@ncl.ac.uk > > > > > -- > > *From:* CCP4 bulletin board on behalf of Alan > Cheung > *Sent:* 21 June 2020 08:12 > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* [ccp4bb] Cold cabinet recommendations for AKTA Pure > > > > ⚠ External sender. Take care when opening links or attachments. Do not > provide your login details. > > Dear All > > > > Apologies for being wildly off topic, but we're trying to find a decent > cold cabinet for 2x AKTA Pure + F9C + sample pump. Does anyone have any > recommendations, especially for sliding door models? > > > > Cheers, > > Alan > > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > > > -- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 > To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] AW: [ccp4bb] Cold cabinet recommendations for AKTA Pure
Hi Alan, I think new AKTA collector can be refrigerated. If this is not an option, consider putting only collector in the cold cabinet and cooling down the buffers. Also, you can put only columns and fraction collector inside cabinet which has side port for such use. Although you will have to use small diameter out tubing to avoid sample dilution when use like this. System live will be shorter inside cold cabinet due to increased humidity. Michal On Jun 21, 2020, at 11:10, Hughes, Jonathan wrote: CAUTION: This email originated from outside of the University of Saskatchewan. Do not click links or open attachments unless you recognize the sender and know the content is safe. If in doubt, please forward suspicious emails to phish...@usask.ca hi alan, why do you want to put the thing in a cold cabinet? if you have protease problems, there are better ways to get rid of them than exploiting Q10. on the other hand, you'll increase viscosity and thus slow down the flow rates. also, the high humidity will reduce the lifetime of your Äkta dramatically (ours has been running almost continuously for 20 years at room temperature and is still going strong [thanks to tina!]). if you really to need to keep the sample at 4 °C, you can put the buffer(s) on ice and cool the column with a jacket. best jon Von: CCP4 bulletin board Im Auftrag von Paula Salgado Gesendet: Sonntag, 21. Juni 2020 18:50 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] Cold cabinet recommendations for AKTA Pure Hi Alan We got one a few years back, from Fisher, although I think Thermo are the manufacturer's: Refrigerator FRCR4504W Forma chromatography THERMO SCIENTIFIC FORMA FRCR4504W They also arranged for a heavy metal shelf to hold the AKTA as the normal shelves won't hold the weight. We only have one AKTA Pure there, with a fraction collector and the rest of the cabinet holds all the columns. Hope that helps Paula === Dr Paula S. Salgado Senior Lecturer in Macromolecular Crystallography Molecular Mechanisms of Life Lead During the current COVID-19 crisis, my working hours tend to focus on early afternoons and evenings, so please bear with me if my reply is delayed and if I email outside your normal working hours. In any case, I do not expect a response outside those hours. Stay home if you're not involved in essential work, stay safe. Newcastle University Biosciences Institute Faculty of Medical Sciences 2nd Floor Cookson Building Newcastle University Newcastle upon Tyne, NE2 4HH, UK Tel: +44 (0)191 208 7432 Email: paula.salg...@ncl.ac.uk<mailto:paula.salg...@ncl.ac.uk> From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK>> on behalf of Alan Cheung mailto:alan.che...@ucl.ac.uk>> Sent: 21 June 2020 08:12 To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: [ccp4bb] Cold cabinet recommendations for AKTA Pure ⚠ External sender. Take care when opening links or attachments. Do not provide your login details. Dear All Apologies for being wildly off topic, but we're trying to find a decent cold cabinet for 2x AKTA Pure + F9C + sample pump. Does anyone have any recommendations, especially for sliding door models? Cheers, Alan To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] AW: [ccp4bb] Cold cabinet recommendations for AKTA Pure
hi alan, why do you want to put the thing in a cold cabinet? if you have protease problems, there are better ways to get rid of them than exploiting Q10. on the other hand, you'll increase viscosity and thus slow down the flow rates. also, the high humidity will reduce the lifetime of your Äkta dramatically (ours has been running almost continuously for 20 years at room temperature and is still going strong [thanks to tina!]). if you really to need to keep the sample at 4 °C, you can put the buffer(s) on ice and cool the column with a jacket. best jon Von: CCP4 bulletin board Im Auftrag von Paula Salgado Gesendet: Sonntag, 21. Juni 2020 18:50 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] Cold cabinet recommendations for AKTA Pure Hi Alan We got one a few years back, from Fisher, although I think Thermo are the manufacturer's: Refrigerator FRCR4504W Forma chromatography THERMO SCIENTIFIC FORMA FRCR4504W They also arranged for a heavy metal shelf to hold the AKTA as the normal shelves won't hold the weight. We only have one AKTA Pure there, with a fraction collector and the rest of the cabinet holds all the columns. Hope that helps Paula === Dr Paula S. Salgado Senior Lecturer in Macromolecular Crystallography Molecular Mechanisms of Life Lead During the current COVID-19 crisis, my working hours tend to focus on early afternoons and evenings, so please bear with me if my reply is delayed and if I email outside your normal working hours. In any case, I do not expect a response outside those hours. Stay home if you're not involved in essential work, stay safe. Newcastle University Biosciences Institute Faculty of Medical Sciences 2nd Floor Cookson Building Newcastle University Newcastle upon Tyne, NE2 4HH, UK Tel: +44 (0)191 208 7432 Email: paula.salg...@ncl.ac.uk<mailto:paula.salg...@ncl.ac.uk> From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK>> on behalf of Alan Cheung mailto:alan.che...@ucl.ac.uk>> Sent: 21 June 2020 08:12 To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: [ccp4bb] Cold cabinet recommendations for AKTA Pure ⚠ External sender. Take care when opening links or attachments. Do not provide your login details. Dear All Apologies for being wildly off topic, but we're trying to find a decent cold cabinet for 2x AKTA Pure + F9C + sample pump. Does anyone have any recommendations, especially for sliding door models? Cheers, Alan To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
Re: [ccp4bb] Cold cabinet recommendations for AKTA Pure
Hi Alan We got one a few years back, from Fisher, although I think Thermo are the manufacturer's: Refrigerator FRCR4504W Forma chromatography THERMO SCIENTIFIC FORMA FRCR4504W They also arranged for a heavy metal shelf to hold the AKTA as the normal shelves won't hold the weight. We only have one AKTA Pure there, with a fraction collector and the rest of the cabinet holds all the columns. Hope that helps Paula === Dr Paula S. Salgado Senior Lecturer in Macromolecular Crystallography Molecular Mechanisms of Life Lead During the current COVID-19 crisis, my working hours tend to focus on early afternoons and evenings, so please bear with me if my reply is delayed and if I email outside your normal working hours. In any case, I do not expect a response outside those hours. Stay home if you're not involved in essential work, stay safe. Newcastle University Biosciences Institute Faculty of Medical Sciences 2nd Floor Cookson Building Newcastle University Newcastle upon Tyne, NE2 4HH, UK Tel: +44 (0)191 208 7432 Email: paula.salg...@ncl.ac.uk From: CCP4 bulletin board on behalf of Alan Cheung Sent: 21 June 2020 08:12 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Cold cabinet recommendations for AKTA Pure ⚠ External sender. Take care when opening links or attachments. Do not provide your login details. Dear All Apologies for being wildly off topic, but we're trying to find a decent cold cabinet for 2x AKTA Pure + F9C + sample pump. Does anyone have any recommendations, especially for sliding door models? Cheers, Alan To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
[ccp4bb] Cold cabinet recommendations for AKTA Pure
Dear All Apologies for being wildly off topic, but we're trying to find a decent cold cabinet for 2x AKTA Pure + F9C + sample pump. Does anyone have any recommendations, especially for sliding door models? Cheers, Alan To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
