Well - if the CCs are 0 then no averaging can take place.
You know you can let DM make the mask itself - are you using the GUI?
It shows you what to set..
Eleanr
zhan...@umbc.edu wrote:
Hi,
Thanks for reminding me checking the mask. I think their might be
something wrong with the mask,
The commonest error with averaging is getting the mask wrong.
Check that the CCs after application of the averaging start at a
reasonable value - 0.3 at least and increase with each cycle ( by the
way why do ncycle 1?)
But in the end the density will not be identical, the Fobs are not
Hi,
Thanks for reminding me checking the mask. I think their might be
something wrong with the mask, since when DM read in the mask, it says:
Number of columns, rows, sections ... 84 74 69
Map mode 0
Hi,
I am using the following DM script to perform a NCS averaging. I have a
fundemental question: after NCS averaging, are the density distrubitions
of different NCS unit being averaged supposed to be the same? I found they
are different by checking FCDM/PHICDM, and maybe I am wrong somewhere...
Regarding your script:
change a couple of things:
MODE HIST SOLV MULT AVER
COMBINE PERT
SCHEME RES FROM 3.0 (or from where you have FOM 70%, as your low res phases
will be more reliable)
NCYCLE 50 (since you have 12 molecules you should get a significant benefit
from averaging.
Then the
Thanks. I will try the comprehensive script. Originally I just wanted to
see what happens if I do NCS averaging only without solvent flattening or
histogram match, to see whether all NCS units are the same. Anyway, it is
always better to use more of them.
Also thanks for pointing out that the
