Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

In my case what simply worked is to put your solution with 40% glycerol on the side of the drop and wait for crystals to stop dancing. Fish them out swiping through the side were you have put your solution. Or I have used MPD as cryoprotectant and freeze them in cold room (4C) its amazing what c

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

Ahh, yes, the "dancing crystals problem".  The good news is alcohols are really good cryoprotectants as well as excellent precipitants. Shame their use has fallen out of favor over the years, but I guess as drop sizes got smaller the evaporation problems got worse and worse. In my humble opi

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

Max-von-Laue-Strasse 3 > 60438 Frankfurt / Main > Germany > > Office +49 69 6303 1056 > Lab +49 69 6303 1074 > Fax +49 69 6303 1002 > > Am 15.08.2018 um 08:05 schrieb Kajander, Tommi A: > > Yes sorry, i meant paratone-N also. > > > > Tommi > >

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

, i meant paratone-N also. Tommi Kohteesta: ferrer Lähetetty: keskiviikko 15. elokuuta klo 0.41 Aihe: Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant Vastaanottaja: ccp4bb@jiscmail.ac.uk Dear Thomas, Alternatively you can try shooting on crystals in the drop, in situ. So

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

Yes sorry, i meant paratone-N also. Tommi Kohteesta: ferrer Lähetetty: keskiviikko 15. elokuuta klo 0.41 Aihe: Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant Vastaanottaja: ccp4bb@jiscmail.ac.uk Dear Thomas, Alternatively you can try shooting on crystals in the drop, in

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

Dear Thomas, Alternatively you can try shooting on crystals in the drop, in situ. So fishing, no cryo. But potentially high radiation damage. Can be considered if you have enough crystals, and if your crystallization plate makes it possible. Regards JL On 14/08/2018 20:58, Thomas Krey wrot

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

I just give an example of oil. Indeed, paraffin is also a very good option. In some cases (<=5% alcohol). Here is the difference I observed between paraffin and paratone oil. 1. Paraffin oil has low viscosity. Paratone oil is too sticky with high viscosity. Sometimes, I mix them with different rat

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

I second (third?) what Tommi and Kevin said about using an oil to cover the drop to slow evaporation (I like paraffin for this—not too viscous). Here’s an additional nuance: Saturate the oil with the alcohol first, before using it to cover the drop. > On 14 Aug 2018, at 2:58 PM, Thomas Krey w

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

Hi Thomas, This is usual when high volatile solvent is used in crystallization (membrane or glycoproteins). The crystal may looks very nice with sharp edges. When you open the cover glass, you may see a very thin film formed on the hang-on drops. Once you touch the drop, then crystals move very qu

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

Hi, you could try picking in the cold room. Provided the temperature change does not kill the crystals, this sometimes worked fine for me in similar cases. Petri Petri Kursula -- Professor Department of Biomedicine University of Bergen, Norway http://www.uib.no/en/rg/petrikursula

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

> Subject: [ccp4bb] Fishing crystals from volatile solvent as precipitant CAUTION: This message originated from a non UMB, UMSOM, FPI, or UMMS email system. Whether the sender is known or not known, hover over any links before clicking and use caution opening attachments. Dear crystallization exper

Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant

@JISCMAIL.AC.UK Subject: [ccp4bb] Fishing crystals from volatile solvent as precipitant CAUTION: This message originated from a non UMB, UMSOM, FPI, or UMMS email system. Whether the sender is known or not known, hover over any links before clicking and use caution opening attachments. Dear

[ccp4bb] Fishing crystals from volatile solvent as precipitant

Dear crystallization experts, We have 3D protein crystals grown from a microseed matrix screening vapor diffusion experiment in either 15% (v/v) Reagent alcohol HEPES Na pH 7.5 0.2 M MgCl2 or in 27% Isopropanol 0.18 M MgCl2 90 mM HEPES Na pH 7.5 10% Glycerol Upon opening the corresponding wel