Hi Urmi,
When you say antibody you mean Fab fragments? If so, bear in mind that
Fab fragments can be quite flexible about the region inbetween the
variable and constant domains, which may be detrimental to the quality of
your crystals ... in this case, further to the advice of others on here,
you
!
Herman
-Ursprüngliche Nachricht-
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Urmi
Dhagat
Gesendet: Freitag, 24. Mai 2013 04:21
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] Improve diffraction ...any ideas?
Hi,
I am working on a protein antibody complex which
Use random microseeding to pick up new conditions, and work with those.
See http://www.douglas.co.uk/mms.htm and
http://www.douglas.co.uk/MMS_proc.htm for theory, references and practical
details
On 24 May 2013 03:20, Urmi Dhagat udha...@svi.edu.au wrote:
Hi,
I am working on a protein
Rajiv, I don't quite get your idea. Once the crystals of the single
proteins have grown, you can't soak the other protein in, can you? Or do
you mean something else?
Umri, if you do get crystals of one of the components it's well worth
trying cross-seeding into the complex, again using random
I think this is an advice not to follow.
Vaheh
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Rajiv K
Bedi
Sent: Friday, May 24, 2013 1:44 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Improve diffraction ...any ideas?
Dear Umri
I think you can soak another protein into a protein crystal if it is small
enough to pass through water channels, I guess.
More info:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2483499/
Hello Scott
Setting up rMMS by hand works fine, but it's a bit slow and uses more
protein and (sometimes much more important!) more seed stock.
We recommend using a Hamilton syringe, preferably with a rounded needle, to
set up by hand. 1.0 protein + 0.7 reservoir solution + 0.3 seed stock
works
Dear Umri,
I think the main problem is co-crystallization.
What I would do is crystallize protein and antibody separately and then soak
protein crystals into reservoir solution containing antibody or vice versa.
And do try to get crystals from different conditions which may alter the space
