Thank you for your comments everyone.
The CCP4BB is a wonderful resource and it has answered several questions
that have been bothering me for years!
Tristran has given us the correct conclusion as well as the important
facts: the capacity of oil for holding O2 is high, but the diffusion rate
is
Do you have evidence that the oil blocks diffusion of O2? O2 is a nonpolar
molecule, generally much more soluble in oils than in water. I'm not sure about
silicone oils, but I would think they also dissolve O2 readily.
eab
On 03/18/2015 08:02 AM, Patrick Shaw Stewart wrote:
Hi Steve
I have
Hi Steve
I have one more comment for this thread.
The microbatch-under-oil method is very handy for anaerobic work:
1. You can keep the microbatch stock solutions in normal microtitre plates
(polypropylene is best to reduce evaporation) for months, which hugely
reduces the amount of degassing
Hi,
As far as I can tell oil does not block diffusion of O2 whatsoever. You
can keep larger volumes (≥1 ml) of solutions anoxic in air for several
hours with dithionite (≥0.5%) to scavenge oxygen and a redox indicator
dye such as phenosafranin to monitor the state of the solution. Small
I also wondered about the statement about oils blocking diffusion of O2. We
had lots of trouble keeping things anaerobic in a glove box until we degassed
the oils and waxes used to mount crystals in capillaries. We found that
putting them under vacuum removed much of the dissolved oxygen.
It's a little complicated. It's true that oxygen is more soluble in most oils
than in water - but in a high viscosity mineral oil the diffusion rate is
orders of magnitude lower. So the combination of an oil overlay and a reducing
agent in your buffer should protect your sample much longer than
Actually I may have misunderstood the original post. Patrick never said oils
block O2 diffusion:
On 03/18/2015 09:47 AM, Edward A. Berry wrote:
The microbatch-under-oil method is very handy for anaerobic work:
(In a glove box, of course)
1. You can keep the microbatch
Hi Stephen,
we crystallized several proteins in a glove box placed in a room with
air condition. We checked the temperature all the times and found that
is had been quite stable.
For one protein that crystallized only at 4 deg C, we setup the
crystallization plate within the glove box and
2015 10:17
To: ccp4bb
Subject: [ccp4bb] Off-topic - Crystallisation in anaerobic glove box
Dear CCP4BBer's
Apologies for the off-topic post, but the CCP4BB seems to be the best place to
ask about crystallisation.
I am looking to set up crystallisation in an anaerobic glove box and wondered
how
Dear CCP4BBer's
Apologies for the off-topic post, but the CCP4BB seems to be the best place to
ask about crystallisation.
I am looking to set up crystallisation in an anaerobic glove box and wondered
how other people did this, specifically the crystallisation stage. My initial
thoughts were
Dear Steve,
There is a model of small incubator that fits through the large portal of a
Belle Technology rigid glovebox - as an aside, that’s a good box for
crystallisation and crystal manipulation since you can get the
microscope-mounted side box with a dewar port for rapid and pretty
Dear Steve,
You should not require an incubator if you have good, stable air con. We
routinely grow crystals in plates stored at room temperature in wall mounted
cabinets in our crystallisation lab. Another of the groups here have
experience of growing crystals in an anaerobic box.
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