al message --
>From: Ngo Duc Tri <[EMAIL PROTECTED]>
>To: [EMAIL PROTECTED]>
>Reply to: Ngo Duc Tri <[EMAIL PROTECTED]>->
>Subject: [ccp4bb] Optimization of needle crystals?
>
> Dear ccp4 users,
> I crystallized a membrane protein and got some c
You might have to test if it is salt/detergent or protein crystal first. If
it is really protein crystal, never give up and for needle crystal,
generally it is difficult to optimze, however in most cases, the diffraction
is well enough. If it is protein crystal, as some researcher has mentioned,
fi
Dear Ngo,
You could try additive screens, such as those sold by Hampton Research.
Using an additive identified during screening, I was able to optimize
conditions giving thin, fiber-like needles to get chunky plates. This was
with a soluble protein, though. I don't know whether this approach will
letin board on behalf of Ngo Duc Tri
Sent: Wed 4/16/2008 9:48 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Optimization of needle crystals?
Dear experts,
You didn't mention which detergent in the conditions. From the
picture it looks
like crystals of detergent.
The initial buffer c
PROTECTED]>
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Van Den Berg, Bert
Sent: Wednesday, April 16, 2008 9:09 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Optimization of needle crystals?
Hi Ngo,
your needles actually look like very
08 856 1211 (lab)
> e-mail: [EMAIL PROTECTED]://www.umassmed.edu/pmm/faculty/vandenberg.cfm
>
>
> --
> *From:* CCP4 bulletin board on behalf of Ngo Duc Tri
> *Sent:* Wed 4/16/2008 9:48 AM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] Optimization of needle crystals?
&
CTED]
http://www.umassmed.edu/pmm/faculty/vandenberg.cfm
From: CCP4 bulletin board on behalf of Ngo Duc Tri
Sent: Wed 4/16/2008 9:48 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Optimization of needle crystals?
Dear experts,
You didn'
You may be able to check whether the cyrstals are protein or detergent
this using powder diffraction. (please excuse the shameless plug):
Acta Cryst. (2008). A64, 169-180 "Powder crystallography on macromolecules"
I. Margiolaki and J. P. Wright
http://journals.iucr.org/a/issues/2008/01/00/sc5011
You didn't mention which detergent in the conditions. From the picture it looks
like crystals of detergent.
--
Hubing Lou
Centre for Biomolecular Sciences
University of St Andrews
North Haugh
St Andrews
Fife
KY16 9ST
Scotland
Quoting Ngo Duc Tri <[EMAIL PROTECTED]>:
> Dear ccp4 users,
> I cry
