Re: [ccp4bb] Secrets of ZYMIT

2019-09-13 Thread mesters
Besides detergents and I do not know what secret ingredients, the mixture includes *two* proteolytic enzymes. If I were to design the mixture for needle cleaning, to digest different types of proteins, I would probably not use a costly enzyme with high substrate specificity, I would use

Re: [ccp4bb] Secrets of ZYMIT

2019-09-09 Thread Jorg Stetefeld
Rupp Sent: Monday, September 9, 2019 5:00 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Secrets of ZYMIT Well, trade secret means exactly what it says. We were equally unable to extort it from the suppliers. MS fragment search as suggested might work. Best, BR From: CCP4 bu

Re: [ccp4bb] Secrets of ZYMIT

2019-09-09 Thread Bernhard Rupp
: [ccp4bb] Secrets of ZYMIT Hi everyone, this is Joerg Stetefeld. I would like to approach the community in a peculiar case of a specific proteolytic cleavage during crystallization attempts. Performing several crystallization screens we figured out that ZYMIT (https://www.ipcol.com

Re: [ccp4bb] Secrets of ZYMIT

2019-09-08 Thread Bonsor, Daniel
board on behalf of Jorg Stetefeld Sent: Sunday, September 8, 2019 6:01:49 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Secrets of ZYMIT Hi everyone, this is Joerg Stetefeld. I would like to approach the community in a peculiar case of a specific proteolytic cleavage during crystallization at

[ccp4bb] Secrets of ZYMIT

2019-09-08 Thread Jorg Stetefeld
Hi everyone, this is Joerg Stetefeld. I would like to approach the community in a peculiar case of a specific proteolytic cleavage during crystallization attempts. Performing several crystallization screens we figured out that ZYMIT (https://www.ipcol.com/cleaners/zymit-low-foam) is the