Hi Giulliana,
What is the percent identity of your best search model with your target? At
what resolution does your crystal diffract?
If you use PHASER without choosing the space group, it might help find you
a solution in a different space group than you expect.
There are also some tricks on
Hi everyone,
I'd like to add that tunable synchrotron beamlines are tending more and more
to support Sufur-SAD on native proteins - in particular, there are dedicated
long-wavelength beamlines (like I23 at Diamond).
And if radiation damage is the main concern, availability of multiple crystals
Dear all,
I would like to known if someone could help me with some idea about the
phase problem.
I am a beginner in crystallography and the first time I tryed to solve the
structure by Molrep, amore, mr. Bump and I didnt find anything for
molecular replacement.
Thus, currently I've tried to
Screen for heavy atoms with native gel. Run protein with variety of heavy
atoms. If you see a discreet shift in the native gel, then you will get
derivative. If you don't see a shift, don't bother soaking. It's worked
for me every time. Also, ammonium sulfate will mess up your heavy atoms,
so
Hi Giuliana,
if your crystal diffracts to 3 Å or better and you have at least one
Cys/Met per 25 residues, put it on the home source and collect until the
sulfur signal rises above the noise. Then solve by SAD.
http://www3.imperial.ac.uk/xraycrystallography/learning/sulfur_sad
Andreas
Hi Giulliana,
I would (re)check for issues during the assignment of the space group prior
to MR. Many people in the list can help you with this if you provide
additional information regarding your data.
In case you have a structure that can not be solved by MR, I would give a
chance for the
