Dear All,
I have a postdoc vacancy in my lab at EMBL Heidelberg, Germany.
The ideal candidate should have some experience in crystallography and be
enthusiastic to discover molecular mechanisms of complex nucleic acid -
protein machines (transposases and/or small RNA mediated silencing
Hi Mahesh,
TESTGEN START 1 END 120 ANGLE 0.5
moving the detector further back or using 2theta and collecting more frames.
When you started shooting at your image you should have checked for overlaps
ahead of time.
I assume this to about 1.8 Å - maybe it would have been wiser to collect a 2.5
Å
doesn't necessarily looked twinned to me.
Rather it looks like you have a trigonal or hexagonal cell with a long c-axis.
On image 2 it seems you may have systematic absences along l, although it is
hard to tell the order. Perhaps P31, P32, P62, P64 or spacegroups with these
symmetries plus
PS For future data collections, try to get the long c-axis roughly along the
crystal rotation axis if possible.
On 16 Aug 2013, at 16:38, Mahesh Lingaraju wrote:
Hi CCP4 folks
I have a data set which is looks twinned ( see the image-1 - I zoomed on to
the image so that one can spot the
Hi Mahesh
In addition to what Mark and Juergen have written, it looks to me like you have
very high mosaicity in one direction - the first image shows no distinct lunes;
I suspect that may have something to do with the failure to process - but what
do you mean when you say you cannot process
This is clearly a case of a crystal with a very long unit cell; a case
which should be approached mindfully.
HKL2000 has a default search for indexing solutions such that diffraction
along the longest unit cell will be resolved, with the assumed spot size.
The problem with such diffraction has 2
for #2)
I'd suggest get some of those Mitigen loops that are titled. I assume you have
hexagonal plates as crystals and you really want to shoot along the thin area
of the crystal down the sixfold. With normal loops it's an art to get that
crystal to sit upright in the loop but not impossible
This is clearly a case of a crystal with a very long unit cell; a case
which should be approached mindfully.
HKL2000 has a default search for indexing solutions such that diffraction
along the longest unit cell will be resolved, with the assumed spot size.
The problem with such diffraction has 2
This is continuation of the previous message, which was sent prematurely.
In case of crystals with one, very long unit cell data collection strategy
needs to be carefully chosen.
Ad. 2) What would be a better way to collect data in the future?
First, the detector needs to be placed far back
tilted is what I meant at an angle of e.g. 30 or 60 degrees. Works fine with
most SSRL beamlines except of the 12-2 microfocus - but that might have been
fixed in the meantime.
Jürgen
On Aug 16, 2013, at 1:57 PM, Bosch, Juergen wrote:
for #2)
I'd suggest get some of those Mitigen loops that
Dear All,
The purpose of statistics in the output of Scalepack is to help the
experimenter to assess the data. The question is, what is the purpose of
R-merge statistics and its usefulness when its value exceeds 100%?
When Scalepack was originally written 20 years ago, I made a decision to
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