Re: [ccp4bb] high SOLVENT content low RESOLUTION and a LIGAND to be found

2018-11-21 Thread Eleanor Dodson
What is the cell difference? Space group difference- surely not??? Between the two data sets. At lowish resolution the maps can tolerate more cel difference of course than at high resolution.. Eleanor On Wed, 21 Nov 2018 at 13:09, Almudena Ponce Salvatierra < maps.fa...@gmail.com> wrote: > Dear

[ccp4bb] high SOLVENT content low RESOLUTION and a LIGAND to be found

2018-11-21 Thread Almudena Ponce Salvatierra
Dear all, I have recently determined the structure of a protein for which useful data extends to 4.2 angstroms (as assessed by paired refinement after pdb redo), with some dazzling 80% solvent content and... I am expecting to find a 26-atom ligand bound to it. However the "blobbyness" of the maps

Re: [ccp4bb] high SOLVENT content low RESOLUTION and a LIGAND to be found

2018-11-21 Thread Pavel Afonine
Hi Almudena, I wonder to which extent I can trust the positive blobs or polder maps that > I am generating... > the answer to this question is given in corresponding paper that describes Polder map: http://journals.iucr.org/d/issues/2017/02/00/ba5254/ba5254.pdf Based on the analysis described

Re: [ccp4bb] high SOLVENT content low RESOLUTION and a LIGAND to be found

2018-11-21 Thread Almudena Ponce Salvatierra
Dear Pavel, thanks for the quick reply. Indeed, when I place the ligand where I suspect it could be, the program says: The polder map is likely to show the ligand. Indeed I see a blob ... I watched the video tutorial some time ago. It's very easy to use, indeed. Concerning the Fobs-Fobs maps,

[ccp4bb] cryo-EM training videos

2018-11-21 Thread Jensen, Grant J.
Dear colleagues: For those of you interested in cryo-EM, Matthijn Vos and I would like to announce that we have just made a large set of new training videos public covering the operation of Arctica and Titan Krios microscopes. The videos are part of an ongoing expansion of the original

[ccp4bb] Postdoctoral position at ODDI & SGC Oxford

2018-11-21 Thread Tobias Krojer
Dear all, We have an opportunity for a Postdoctoral Scientist with an interest in protein crystallography and fragment-based discovery to join the group of Prof Paul Brennan, head of Chemistry at the ARUK Oxford Drug Discovery Institute (ODDI) and the Structural Genomics Consortium (SGC). The