Re: [ccp4bb] database of crystallization condition

--- Bostjan Kobe NHMRC Research Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research Cooper Road University of Queensland Brisbane, Queensland

Re: [ccp4bb] Maltose binding protein as a tag

Dear Kostas There is a chance your protein is not properly folded by is solubilized by the large MBP tag, and this may be the reason for aggregation. Bostjan --- Bostjan Kobe NHMRC Research Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute

[ccp4bb] Post-doctoral positions in protein crystallography, University of Queensland, Brisbane, Australia

POST-DOCTORAL POSITIONS IN PROTEIN CRYSTALLOGRAPHY, UNIVERSITY OF QUEENSLAND, BRISBANE, AUSTRALIA Applications are invited for two protein crystallography post-doctoral positions in the laboratory of Prof Bostjan Kobe at the School of Molecular and Microbial Sciences (SMMS) and the Institute

Re: [ccp4bb] His tag

your time because of my inadequate knowledge Thank you for your help Yanming --- Bostjan Kobe ARC Federation Fellow Professor of Structural Biology School of Molecular and Microbial Sciences and Institute for Molecular Bioscience Office: Building 76 Room 452 Cooper Road University of Queensland

Re: [ccp4bb] O/T: can a protein which dimerizes in solution crystallize as a monomer?

right back to your original question. --- Bostjan Kobe ARC Federation Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience Cooper Road University of Queensland Brisbane, Queensland 4072 Australia Phone: +61 7 3365 2132 Fax: +61

[ccp4bb] Postdoctoral position at the University of Queensland, Brisbane, Australia

are invited for a protein crystallography post-doctoral position in the laboratory of Prof Bostjan Kobe at the School of Molecular and Microbial Sciences (SMMS) and the Institute for Molecular Bioscience (IMB), University of Queensland, Brisbane, Australia. The main area of study will involve

Re: [ccp4bb] monomer-dimer

LABORATORY OF STRUCTURAL BIOINFORMATICS KOREA UNIVERSITY, SEOUL --- Bostjan Kobe ARC Federation Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious

[ccp4bb] Postdoctoral position in Brisbane Australia

POSTDOCTORAL POSITION IN MACROMOLECULAR CRYSTALLOGRAPHY, UNIVERSITY OF QUEENSLAND, BRISBANE, AUSTRALIA Applications are invited for a post-doctoral position in macromolecular crystallography in the laboratory of Prof Bostjan Kobe at the School of Chemistry and Molecular Biosciences (SCMB

Re: [ccp4bb] Structure containing nickel ions from Ni-NTA column

Celestijnenlaan 200 F B-3001 Heverlee (Leuven) Tel: +32(0)16327477 -- --- Bostjan Kobe ARC Federation Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience (Division of Chemistry

[ccp4bb] Postdoctoral position in Brisbane Australia

in macromolecular crystallography in the laboratory of Prof Bostjan Kobe at the School of Chemistry and Molecular Biosciences (SCMB) and the Institute for Molecular Bioscience (IMB), University of Queensland, Brisbane, Australia. The main area of study will involve characterization of three

Re: [ccp4bb] Biological assembly

subunits in a biological dimer, for example, may be related by crystallographic axis, but the NCS may be a different non-physiological crystal contact, therefore the ASU won't be the same as the biological dimer. So I am not sure if the above applies in general. Bostjan Bostjan Kobe NHMRC Research

Re: [ccp4bb] Efficient way of showing residue conservation

Consurf will do this for you. Bostjan --- Bostjan Kobe NHMRC Research Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research Cooper

Re: [ccp4bb] Hanging vs. Sitting

-kell...@northwestern.edu *** --- Bostjan Kobe ARC Federation Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience Cooper Road University of Queensland Brisbane, Queensland 4072

[ccp4bb] Postdoctoral position in Brisbane, Australia

OF QUEENSLAND, BRISBANE, AUSTRALIA Applications are invited for two macromolecular crystallography post-doctoral positions in the laboratory of Prof Bostjan Kobe at the School of Chemistry and Molecular Biosciences (SCMB) and the Institute for Molecular Bioscience (IMB), University of Queensland, Brisbane

Re: [ccp4bb] unknown density

with something? Picture is attached. Thanks a lot, Katja __ Do You Yahoo!? Sie sind Spam leid? Yahoo! Mail verfügt über einen herausragenden Schutz gegen Massenmails. http://mail.yahoo.com --- Bostjan Kobe ARC Federation Fellow

Re: [ccp4bb] crystal contacts

as a result of crystal contacts and those that are intrinsic molecular interactions. Paul. --- Bostjan Kobe ARC Federation Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience (Division of Chemistry and Structural

[ccp4bb] Post-doctoral position at the University of Queensland, Brisbane, Australia

Applications are invited for a post-doctoral position in the group of Bostjan Kobe at the School of Chemistry and Molecular Biosciences (SCMB), University of Queensland, Brisbane, Australia. We are searching for a motivated candidate, ideally with experience with cryo-electron microscopy

Re: [ccp4bb] cryptic error message trying to use LigPlot on an unknown ligand

Hi Fred Ligplot diagram is part of PDBsum output - maybe that will work for you. Bostjan -- Bostjan Kobe FAA Australian Research Council Laureate Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience (Division of Chemistry

Re: [ccp4bb] crystallizing fusion proteins

and purifying new constructs. Despite this, other than specific cases like GPCRs, there are not that many successful examples really. So my feeling is this approach doesn’t work that often without a lot of trial and optimization. Best wishes Bostjan -- Bostjan Kobe FAA Australian Research Council

[ccp4bb] PhD scholarship at the University of Queensland Brisbane Australia

and contact Bostjan Kobe at b.k...@uq.edu.au. Please circulate this to potential candidates. The scholarship is open to international students. Best wishes Bostjan -- Bostjan Kobe FAA Australian Research Council Laureate Fellow Professor of Structural Biology School of Chemistry and Molecular

Re: [ccp4bb] Future Diffraction Methods

not feel isolated, despite not being able to participate in every technical discussion on methods I am not familiar with. Bostjan -- Bostjan Kobe FAA Australian Laureate Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience

Re: [ccp4bb] A challenging MR problem

Superimposing that molecule on all the others? Bostjan -- Bostjan Kobe FAA Australian Laureate Fellow Professor of Structural Biology School of Chemistry and Molecular Biosciences and Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Australian Infectious