Dear all -
I have also had success with crystallization above room temperature. I once
grew diffracting crystals of a small, thermostable enzyme by incubating the
plates in a 37°C bacterial culture incubator, and despite similar reservoir
conditions, these high-temperature crystals grew in a d
Dear all,
I'm looking at a crystal structure (1H4G) where the N-terminal Glu residue
has cyclized to pyroglutamic acid (PCA). The protein was expressed in and
secreted from bacteria (*Bacillus licheniformis*), and the crystallization
conditions for 3 ul hanging drops were 2 ul protein solution (1
incipal Research Scientist - Structural Biology
> National Physical Laboratory (NPL)
> Hampton Rd | Teddington | Middlesex | TW11 0LW
> -----------
>
>
> --
> *From:* CCP4 bulletin board on behalf of Jare
Hi Dhiraj - In addition to the other good suggestions, you may also wish to
try codon optimization and different secretion signal peptides if your
target protein is secreted.
Hope that helps.
Cheers,
Jared
On Thu, Jun 24, 2021 at 4:42 PM Srivastava, Dhiraj <
dhiraj-srivast...@uiowa.edu> wrote:
Hi Phoebe -
Your email got me wondering about this as well, so over the weekend I
downloaded BioViewer (simple and relatively intuitive, few customizable
options), and iMolview (more complex user interface, with more
information—e.g. sequence, chain IDs—and more granular control of
representation)
ional protons are present in the
>> model for the positively charged histidines, the residues in question
>> are indicated in both the SEQRES and the ATOM records as 3-letter code
>> `HIS` regardless of protonation state (i.e. instead of `HIP` for
>> positively charged, and `
Hi Dipankar -
I will echo Thuy's suggestion of higher glycerol concentration in your
refolding buffer. A protocol I used in a previous lab (for refolding antibody
ScFv fragments) steps down gradually over 3 days from 10%-5%-0% glycerol in the
dialysis buffer, from an initial sample of inclusi
Hi Sayli -
PrePPI may be helpful if you're interested in interactions among human proteins.
http://honig.c2b2.columbia.edu/preppi/
Cheers,
Jared
Hi Faisal -
In addition to the `specular`, `ray_trace_mode`, and `ray_trace_gain`
settings Blaine pointed out, you can also adjust the `direct` and `ambient`
lighting settings, as well as `light_count` (which I believe only affects
direct and specular lighting, not ambient). For example, these wo
