Re: [ccp4bb] Off Topic- Cystine Detection

2013-02-06 Thread Xavier Brazzolotto
and my results were not as clear as I would have hoped for. I am not a sulfur/cysteine chemist and would appreciate the advice on what experiments to try! Thanks a bunch YAP Xavier Brazzolotto, PhD Département de Toxicologie Institut de Recherche Biomédicale des Armées 24 Av des Maquis du

Re: [ccp4bb] Cell free expression

2013-02-27 Thread Xavier Brazzolotto
I've just read that today (even if it is NMR). http://www.nature.com/nmeth/journal/v9/n8/full/nmeth.2033.html /E. coli/ or insects cells are so easy to grow that you should be able to do your own cell extrats easily. Xavier Le 27/02/2013 19:25, Theresa Hsu a écrit : Dear all Out of

[ccp4bb] JLIGAND OSX issue

2018-12-06 Thread Xavier Brazzolotto
e.java:610) at Env.(T.java:312) ... 1 more I’ve tried to reinstall CCP4, but it did not work Any idea how to solve this problem ? Xavier Brazzolotto, PhD xavier.brazzolo...@chemdef.fr <mailto:xavier.brazzolo...@chemdef.fr> Département de Toxicologie et Risques Chimiques Uni

Re: [ccp4bb] how to insert a ligand into a possible binding pocket of one protein strucure model?

2019-03-30 Thread Xavier Brazzolotto
Do you mean docking ? > Le 30 mars 2019 à 00:11, Zing a écrit : > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 >

Re: [ccp4bb] Issues in new Mac version 10.15

2019-10-15 Thread Xavier Brazzolotto
.org > <mailto:rwert...@fredhutch.org> / 206-667-4066 > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1> Xavier Brazzolotto, PhD xavier.braz

Re: [ccp4bb] insect secretion recommendations?

2020-04-20 Thread Xavier Brazzolotto
gentle to not lyse the > insect cells. Thank you, Gloria > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1> Xavier Brazzolotto, PhD xavier

Re: [ccp4bb] mysterious density

2022-03-13 Thread Xavier Brazzolotto
A MES buffer molecule ? > Le 13 mars 2022 à 18:08, doriano lamba a écrit : > > Glycerol-3-phosphate? > My 2cents > DL > Il 2022-03-13 15:19 Amir Khan ha scritto: >> Hi, >> I wonder whether anyone can advise on the ‘tadpole’ like density. >> The head is currently a phosphate >> and I’ve placed a

Re: [ccp4bb] COOT FLEV error

2022-01-26 Thread Xavier Brazzolotto
C10" ele : C: > ERROR:: (runtime error) in fle_view_with_rdkit(): Failed to get atom index > for atom name " C10"" in residue of type MCY [spec: 1 "A" 165 ""] > > Any help or ideas would be greatly appreciated, thanks in advance, stay safe!

Re: [ccp4bb] mutate Methionine to Norleucine in Coot.

2022-10-26 Thread Xavier Brazzolotto
You can only link a residue (L-peptide) N to another residue (L-peptide) N+1 or N-1 In your case you try to link VAL10 to NLE101… Just change NLE101 to NLE11, then it should work. > Le 26 oct. 2022 à 03:37, Jiang Xu a écrit : > > Hi Garib, >Thank you for your reply. I edited the NLE.cif