I never done such experiments but N-Ethylmaleimide labeling and Mass-Spec could be the solution.
Le 6 févr. 2013 à 17:10, Yuri Pompeu <[email protected]> a écrit : > Dear All, > I am trying to probe the existence of a disulfide bond on the surface of my > protein. > I have attempted Ellman´s and my results were not as clear as I would have > hoped for. > I am not a sulfur/cysteine chemist and would appreciate the advice on what > experiments to try! > Thanks a bunch > YAP Xavier Brazzolotto, PhD Département de Toxicologie Institut de Recherche Biomédicale des Armées 24 Av des Maquis du Grésivaudan 38702 La Tronche Cedex France
